转Bt基因抗虫棉对棉蚜的杀虫剂敏感性及解毒酶系的影响  

Effects of transgenic Bt cotton on the susceptibility to insecticides and detoxification enzyme activities of cotton aphid(Aphis gossypii Glover)

作　　者：张炬红[1] 郭建英 万方浩[1] 夏敬源[2] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100094 [2]农业部全国农业技术推广与服务中心,北京100026

出　　处：《昆虫学报》2006年第6期938-943,共6页Acta Entomologica Sinica

基　　金：国家重点基础研究发展规划"973"项目(2002CB111400);国家"十五"科技攻关重大项目(2004BA516A01);欧盟项目(ICA4-CT-2001-10069)

摘　　要：采用浸叶法,以亲本常规棉作对照,室内测定了吡虫啉和辛硫磷对在转Bt基因棉上取食不同世代棉蚜Aphisgossypii Glover的毒力,并用酶标仪测定了转Bt基因棉对棉蚜酯酶、羧酸酯酶和乙酰胆碱酯酶活力的影响。结果表明,棉蚜对吡虫啉和辛硫磷的敏感性并未受Bt棉的影响,2种杀虫剂对分别取食Bt棉1代和多代(60代以上)的棉蚜与取食亲本常规棉棉蚜的LC50值之间均无显著差异。在抗虫棉GK12上取食1代和21代的棉蚜,其酯酶、羧酸酯酶和乙酰胆碱酯酶的活力与取食亲本常规棉的棉蚜之间差异不显著。可见,转Bt基因棉对棉蚜的杀虫剂敏感性和解毒酶活力无显著影响。The toxicities of imidacloprid and phoxim against cotton aphids fed on transgenic Bt cotton for different generations were measured by leaf-dip bioassay in the laboratory and compared with those on parental cotton variety. Activities of esterase, carboxylesterase and acetylcholinesterase of cotton aphids fed on Bt cotton were detected with microtiter plate reader. The results indicated that the susceptibility of cotton aphids to either imidacloprid or phoxim was not affected by Bt cotton. For both kinds of insecticides, there was no significant difference of LCs0 among cotton aphids fed on Bt cotton for 1 generation, on Bt cotton for over 60 generations, and on parental cotton continuously. For all three detoxification enzymes tested, there was no significant difference of enzyme activities among cotton aphids fed on Bt cotton for 1 and 21 generations, and those on parental cotton. It was so concluded that transgenic Bt cotton had no significant effects on the susceptibility to insecticides and detoxification enzyme activities of cotton aphids.

关 键 词：转BT基因棉 棉蚜 杀虫剂敏感性 解毒酶 乙酰胆碱酯酶 

分 类 号：Q965[生物学—昆虫学]