人胸膜间皮细胞的分离和培养  

Isolation and Culture of Human Pleural Mesothelial Cells

作　　者：王洪峰[1] 曲文秀[1] 刘宏博[1] 何平[1] 李胜岐[1] 

机构地区：[1]中国医科大学附属第二医院(盛京医院)呼吸内科,沈阳110004

出　　处：《细胞生物学杂志》2006年第6期923-926,共4页Chinese Journal of Cell Biology

摘　　要：利用两种取材方法建立人胸膜间皮细胞(HPMC)体外培养模型,一是用胰蛋白酶-EDTA消化法,从人肺脏胸膜及壁胸膜上分离胸膜间皮细胞;二是从胸腔积液中分离胸膜间皮细胞;进行胸膜间皮细胞的培养。用形态学和免疫组化S-P法对培养所得细胞进行鉴定。光镜下可见细胞汇合后呈多角形铺路石样,电镜下可见丰富的微绒毛和内质网,免疫组化染色结果显示表达角蛋白、波形蛋白,而抗VIII因子相关单克隆抗体、抗人白细胞CD45表达阴性;证实为人的胸膜间皮细胞。两种取材方法均可以成功建立间皮细胞体外培养模型,方法学上均有可行性;手术标本所得细胞有较高的细胞纯度,取材上胸腔积液更易于得到。Use two methods to establish human pleural mesothelial cells （HPMCs） culture reproducible model in vitro and compare the advantage and disadvantage of them. Mesothelial cells were isolated from human pleura by trypsin EDTA disaggregation and pleural effusion fluid. HPMCs were identified by morphology and immunohistochemstry： Streptaridin peroxidase conjugated method. Confluent HPMCs appeared multipolar and like cobblestone; Numerous surface microvilli and abundant endoplasmic reticulum were observed under electron microscopy. HPMCs expressed cytokeratin and vimentin; Ⅷ factor associated antigen and CD45 were negative. Two methods achieved success in establishment of reproducible model for culture of HPMCs. Those methods were feasibility in methodology. The cells isolated from human pleura had higher purity quotient. Pleural effusion fluid was easier to obtain.

关 键 词：胸膜 人胸膜间皮细胞 培养 

分 类 号：Q813.1[生物学—生物工程]