CD4^+CD25^+调节性T细胞在HIV/AIDS患者中的作用及其与HIV病毒载量的相关性研究  

Role of CD4^+CD25^+ regulatory T cell in HIV infection/AIDS and the correlation between CD4+CD25+ regulatory T cell and HIV viral load

作　　者：刘水腾[1] 杨桂林[1] 姚红艳[1] 李美忠[1] 徐六妹[1] 李丽雄[1] 王火生[1] 周伯平[1] 

机构地区：[1]深圳市东湖医院,深圳518020

出　　处：《中华实验和临床病毒学杂志》2006年第4期364-366,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的研究HIV感染者/AIDS患者外周血CD4+CD25+调节性T细胞(CD4+CD25+regulatoryTcell,Treg)频率、功能及其临床意义。方法选择31例HIV感染者/AIDS患者和30例健康对照者,采用流式细胞仪检测各组外周血Treg的表型和频率。采取MACS磁珠分选CD4+CD25+T细胞,利用[3H]胸腺嘧啶掺入法检测CD4+CD25+T细胞在特异性HIV抗原刺激下对CD4+CD25-T细胞的增殖影响。结果HIV/AIDS患者组与正常对照组相比较,外周血CD4+CD25+T细胞频率在统计学上差异无统计学意义。与正常对照组比较,HIV感染者外周血CD4+CD25+T细胞频率升高,差异有统计学意义(P<0.01);与正常对照组比较,AIDS患者者外周血CD4+CD25+T细胞频率降低,差异有统计学意义(P<0.0001)。HIVRNA病毒载量与患者外周血CD4+CD25+T细胞数量呈正相关性(P<0.01)。CD4+CD25+T细胞具有抑制HIV特异性的CD4+CD25-T细胞的增殖作用。结论HIV感染者/AIDS患者的细胞免疫功能紊乱,CD4+CD25+T细胞能抑制HIV感染者/AIDS患者的HIV特异性细胞免疫反应,促进HIV病毒复制,与形成持续HIV感染有关。Objective To study of the frequency, phenotype, function and clinical significance of CD4^＋ CD25^＋ regulatory T cell （Treg） in peripheral blood from patients with HIV infection/AIDS. Methods Thirty-one patients with human immunodeficiency virus （HIV） infection/acquired immunodeficiency syndrome （AIDS）and 30 normal subjects （NS） were enrolled in this study. The frequency and phenotype of CD4^＋ CD25^＋ T cells in peripheral blood of the patients and normal subjects were detected by flow cytometry. CD4^＋ CD25^＋ T cells were isolated by magnetic activated cell sorter （MACS） assay, and the effect of CD4^＋ CD25^＋ T cells on CD4 ＋ CD25^- T cell proliferation after HIV antigen stimulation by [^3H] HI thymidine incorporation was investigated. Results The proportion of CD4^＋ CD25^＋ T cells in peripheral blood from patients with HIV infection/AIDS had no significant difference compared with NS. The proportion of CD4^＋ CD25^＋ T cells in peripheral blood from patients with HIV infection were significantly increased compared with NS（ P 〈 0.01 ）. But the Treg in peripheral blood from patients with AIDS were significantly increased in contrast to NS（ P 〈 0.0001 ）. These was a positive correlation between HBV DNA viral load and Treg frequency. Treg could specifically inhibit the proliferation of CD4^＋ CD25^＋ T cell. Conclusions The cell-mediated immune function of patients with HIV infection/AIDS was disordered. CD4^＋ CD25^＋ T cells could specially inhibit cell-mediated immune response and aggravate HIV replication and contribute to oersistent HIV infection.

关 键 词：HIV T淋巴细胞 流式细胞术 RNA 

分 类 号：R512.91[医药卫生—内科学]