中国HIV-1主要流行重组株B/CTat基因第一外显子序列特征分析  

Analysis of Tat exon 1 gene sequence characteristics of HIV-1 B/C recombinant viruses prevalent in China

作　　者：黄海龙[1] 邢辉[2] 马鹏飞[2] 关琪[2] 魏民[2] 洪坤学[2] 陈建平[2] 梁浩[2] 司雪峰[2] 全宇[2] 谢必峰[1] 邵一鸣 

机构地区：[1]福建师范大学生物工程学院,福州350001 [2]中国疾病预防控制中心性病艾滋病预防与控制中心病毒免疫室

出　　处：《中华实验和临床病毒学杂志》2006年第4期390-392,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家973重点基础研究发展计划资助项目(2005CB522903);国家自然科学基金(30070040)

摘　　要：目的研究我国人类免疫缺陷病毒1型(HIV-1)流行重组株B/CTat基因第一外显子变异特点,探讨这些变化对其功能的影响。方法从确诊的HIV-1感染者全血样本中提取基因组DNA,经套式聚合酶链反应(PCR)扩增和测序。使用GCG和Bioedit软件中的程序进行系统进化树和氨基酸变异分析,同时进行网上三级结构预测。结果总计154份样品中CRF07-BC为120份,CRF08-BC为34份,两种亚型有较广泛的分布,CRF07-BC与标准株的平均基因离散率为(5.748±1.352),CRF08-BC与标准株的平均基因离散率为(1.259±1.931)。结论我国流行重组株CRF07-BC比CRF08-BC有较长的流行时间,CRF07-BC与CRF08-BC基因第一外显子氨基酸相比,主要为半胱氨酸富含区和核心区的变化。在这两个区位点变化可能影响Tat与细胞因子如cyclinT1的结合能力,而成为CRF07-BC在我国流行中获得传播优势的原因。Objective To explore the characteristics of HIV-1 B/C recombinant viruses gene variation and its effect on function. Methods Genomic DNA was extracted from the whole blond samples collected from HIV-1 positive patient. Nested PCR was carried out and PCR products were purified and used directly for sequencing. The gene-based phylogenetic trees were constructed by MEGA software and amino acid variations were carried out using Bioedit software. The gene distances were calculated by DISTANCE program in GCG software. The threedimensional structure was forecasted by sending sequence to the SWISS-MODEL. Results Of 154 samples, 120 samples belonged to CRF07-BC, 34 samples belonged to CRF08-BC, beth subtypes distributed widely in China. No changes of breakpoints in Tat exon 1 of CRF07-BC were found. Conclusion The epidemic time of CRF07-BC is longer than that of CRF08-BC, the variations of amino acid between CRF07-BC and CRF08-BC were mainly in cysteine-rich region and core region. These changes may have an effect on the transactivity of tat, and as a reason for CRF07-BC viruses more prevalent in China than CRF08-BC.

关 键 词：HIV-1 序列分析 流行重组模式 

分 类 号：R373[医药卫生—病原生物学]