SARS冠状病毒N蛋白基因的表达及其在SARS诊断中的应用  

Cloning and expression of SARS coronavirus nucleocapsid protein and its application in SARS diagnosis

作　　者：杜培革[1] 王笑英[2] 于建石[3] 安丽萍[1] 吕刚[1] 

机构地区：[1]北华大学临床检验诊断学实验室,吉林132000 [2]吉林省人民医院 [3]中国疾病预防控制中心病毒病预防控制所

出　　处：《中华实验和临床病毒学杂志》2006年第4期399-401,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的获得重组SARS冠状病毒(SARS-CoV)N蛋白抗原,建立特异性诊断SARS病毒感染的免疫学方法。方法大肠埃希菌中表达SARS病毒N蛋白基因,用金属螯合层析纯化N蛋白,建立检测SARS抗体的ELISA方法。结果大肠埃希菌中表达了SARS病毒全长N蛋白抗原,经包涵体洗涤和金属螯和纯化后得到纯度较高的重组蛋白。用重组抗原ELISA检测30名SARS患者抗体全部为阳性,30名正常人血清为阴性,30名发热非SARS患者血清为阴性。结论SARS表达核蛋白可以在大肠埃希菌中得到高效表达,纯化的重组N蛋白具有良好抗原性,可用于检测SARS抗体。Objective To express nuclecapsid protein of SARS virus in E. coli,and establish a specific immunologic diagnostic test for SAP, S virus infection. Methods Full length N protein gene of SARS-CoV was cloned and expressed in E. coli. The recombinant protein was purified by NAT chromatography. An indirect ELISA in which the solid phase was coated with recombinant N protein was used to detect antibodies to SARS virus. Results N protein was highly expressed and purified. The protein reacted with serum of SARS patients, and not with the control serum. Conclusion N protein of SARS virus highly expressed in E. coli has stronger antigencity, and can be used for diagnosis of SARS.

关 键 词：严重急性呼吸综合征 冠状病毒科 核壳蛋白质类 酶联免疫吸附测定 

分 类 号：R373[医药卫生—病原生物学]