高效液相色谱法测定枳实导滞丸中黄芩苷含量  

Dertermination of Baicalin in Zhishidaozhi Wan by HPLC

作　　者：石焱[1] 吴琼[2] 

机构地区：[1]中国人民解放军第210医院药剂科,辽宁大连116021 [2]中国人民解放军空军工程大学门诊部,陕西西安710051

出　　处：《解放军药学学报》2006年第6期474-475,共2页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立高效液相色谱法测定枳实导滞丸中黄芩苷含量的方法。方法Intersil C18分析色谱柱（4．6mmID×250mm，粒径5μm），C18保护柱（4．6mm×5mm，5μm）。流动相：乙腈-水（220：780，含5ml三乙胺，磷酸调pH3．0），流速：1．5ml／min，检测波长：276nm。结果黄芩苷保留时间为10．7min，与相邻峰的分离度〉1．5，理论板数为3500。以峰面积Y对进样浓度X线性回归，黄芩苷回归方程：Y=441385．6X＋3467219．9，r=0．9996，线性范围36．00—240．00μg·ml^-1。黄芩苷平均回收率98．3％，RSD为2．1％（n=9）。结论本方法操作简便，结果准确可靠，可用于枳实导滞丸中黄芩苷的含量测定。Aim To develop an HPLC quantitative method for the determination of Baiealin in Zhishidaozhi Wan. Methods The chromatographic conditions include the column Intersil C18 and a mobile phase ： acetonitrile-water （220： 780, which contains 0.5% triethylamine,was adjusted to pH3.0 by phosphoric acid） . The flow rate was 1.5 ml/min and monitored at 276nm. Results The retain time of Baicalin was 10.7min. The number of theoretical plates calculated by Baicalin peak was 3 500. The regression equation for Baicalin was Y = 441 385.6X ＋ 3 467219.9, r = 0. 9996, and the linear range was 36.00 - 240.0μg·ml^-1. The average recovery of Baicalin was 98. 3%, RSD =2.1% （ n =9）. Conclusion The method was sensitive, quick and accurate for the determination of Baicalin In Zhishidaozhi Wan .

关 键 词：HPLC 枳实导滞丸 黄芩苷 

分 类 号：R917[医药卫生—药物分析学]