HPLC法测定安乃近注射液的含量  

Determination of Metamizole Injection by HPLC

作　　者：庄阳辉[1] 石炳光[1] 

机构地区：[1]福州市第二医院,福州350007

出　　处：《海峡药学》2006年第6期57-58,共2页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法测定安乃近注射液含量的方法。方法采用BDSC18（250mm×4．6mm．5pm）色谱柱，以磷酸盐缓冲溶液-甲醇（70：30）为流动相，流速1．0ml·min^-1检测波长为265nm。结果安乃近在1．225-8．164μg范围内进样量与峰面积里良好的线性关系（r=1．000）；平均回收率为99．6%，RSD为0，5％，n=9。结论本方法结果准确．重现性较好。OBJECTIVE To establish a method for determination content of metamizol injection by HPLC. METHODS BDSC18 column was used and 0.05 mol · L^-1 potassium dihydrogen methanol （70 ： 30） was used as mobile phase,the flow rate was 1.0 ml · min^-1 ,the detecting wavelength was at 265 nm. RESULTS There was a good linear relationship between the concentration of metamizol and peak area within a range of 1. 225-8. 164μg,r=1. 000. The average recover was 99.6% with RSD=0. 5%（n=9）. CONCLUSION The method is highly repeatable and accurate.

关 键 词：含量 安乃近注射液 HPLC 

分 类 号：R929.2[医药卫生—药学]