固相小柱对沙苑子总黄酮萃取方法的研究  

Study on the Extraction Method of Total Flavonoids of Astragalus complanatus R.Br.by Solid Phase Cartridges

作　　者：牟英[1] 彭少平[1] 顾振纶[1] 

机构地区：[1]苏州中药研究所苏州大学基础医学系药理教研室,江苏苏州215000

出　　处：《中国野生植物资源》2006年第6期55-57,共3页Chinese Wild Plant Resources

摘　　要：目的:以沙苑子总黄酮为研究对象,考察固相萃取小柱的洗脱条件,确定固相萃取沙苑子总黄酮的实验方法。方法:以不同浓度的甲醇对不同填料的固相小柱进行洗脱,将洗脱液在岛津VP-ODS(150 mm×4.6 mm,5μm)柱上进行高效液相色谱分析。通过比较不同浓度及体积甲醇的萃取效率,确定沙苑子苷在固相萃取小柱上的洗脱条件。结果:用正相氨基小柱以50%甲醇洗脱效果好,回收率可达100%。结论:经试验,正相氨基小柱对沙苑子苷的萃取回收率约为100%,适用于沙苑子总黄酮中沙苑子苷的分析。Objective：To optimize the solid phase extraction method with total flavonids in Astragalus complanatus R. Br. Mothod ： After extracted by different sokd phase cartridges with different content method elution, the elutin was analyzed on a Shimadzu VP- ODS HPLC column. From the recovery achived, the suitable content and volume of elution was found. Result：This method consists of using 10 mL of 50% methanol as the elutin. Between the two kinds of solid phase being tested, normal phase cartridge was found to be the best one. Conclusion：The average extraction recovery of the normal phase cartridges was about 100%. It can be used in the analysis of total flavonoids in Astragalus complanatus R. Br.

关 键 词：固相萃取 沙苑子总黄酮 HPLC 

分 类 号：R284.2[医药卫生—中药学]