Livinα在膀胱移行细胞乳头状瘤中的表达  

Study on the Expression of Livinα Gene in Bladder Papillary Epithelioma

作　　者：鹿占鹏[1] 王卫国[1] 

机构地区：[1]济宁医学院附属第一人民医院

出　　处：《济宁医学院学报》2006年第4期10-11,共2页Journal of Jining Medical University

摘　　要：目的研究膀胱癌相关基因Livin基因在膀胱移行细胞乳头状瘤组织中的表达,研究抑癌基因P53基因在膀胱乳头状瘤组织中突变,研究Livin表达与P53基因突变的关系。方法14例膀胱移行细胞乳头状瘤组织,设计Livinα引物,以半定量RT-PCR的方法,检测膀胱癌组织LivinαmRNA,以免疫组化法检测癌组织P53蛋白。并以10例正常膀胱粘膜作为对照。结果14例膀胱乳头状瘤组织,7例检测到LivinαmRNA表达,10例正常膀胱粘膜组织未检测到LivinαmRNA表达,LivinαmRNA在膀胱移行细胞癌组织中表达率高于正常膀胱粘膜,有统计学意义(P<0.05)。6例膀胱乳头状瘤组织中P53蛋白阳性。4例瘤组织中LivinαmRNA和P53蛋白均阳性。结论Livinα和P53基因可能在膀胱移行细胞乳头状瘤发生发展过程中起协同作用。Objective To investigate the Livind and the P53 gene expression in bladder papillary epithelioma, with emphasis on the relationship between the two genes in the pathogenesis of the neoplasm. Methods Reverse transcription polymerase chain reaction （RT- PCR） and immunohistochemical staining were used respectively to detect the Livin α mRNA and the P53 protein expression in 14 cases of bladder papillary epithelioma, together with 10 normal bladder tissues. Results Of the total 14 cases of bladder papillary epithelioma, Livin α mRNA expression was tested in 7 cases,while no Livin α mRNA expression was appeared in 10 normal bladder tissue. The expression rate of Livin αmRNA in bladder papillary epithelioma was higher than that in normal bladder tissue （P〈0.05）. Meanwhile, P53 protein expression was found in 6 cases of bladder papillary epithelioma. Of note, both Livin α mRNA and P53 protein expression were simultaneously demonstrated in 4 cases of bladder papillary epithelioma. Conclusion The Livin α and P53 gene may play a synergistic role in the initiation and the progression of bladder papillary epithelioma.

关 键 词：膀胱乳头状瘤 LIVIN P53 RT—PCR 

分 类 号：R697[医药卫生—泌尿科学]