小细胞团为受体的根癌农杆菌介导的非洲菊基因转化体系  

A NEW TRANSGENIC SYSTEM OF GERBERA MEDIATED BY AGROBACTERIUM TUMEFACIENS

作　　者：叶华[1] 陈善娜[2] 陈苇[3] 李劲峰[3] 李根泽[3] 王敬乔[3] 

机构地区：[1]思茅师范高等专科学校生物系,云南思茅665000 [2]云南大学生命科学学院,云南昆明650221 [3]云南省农业生物技术重点实验室云南省农业科学院经济作物研究所,云南昆明650223

出　　处：《西南农业大学学报（自然科学版）》2006年第6期1023-1026,共4页Journal of Southwest Agricultural University

基　　金：云南省"十五"攻关资助项目(2001NG19);省院省校合作资助项目(2000YZ01);人培资助项目(2005PY-01-13)

摘　　要：以非洲菊叶柄基部的愈伤组织为起始材料,通过匀浆将其切割成小细胞团,再利用该小细胞团为外植体进行农杆菌介导的GUS基因转化。标记基因GUS的转化及转化愈伤组织的X-G luc染色证明了该方法可以高频率地(>80%)获得转化愈伤组织;通过对转化愈伤组织的再生培养,获得了8株X-G luc染色阳性植株;转化植株的PCR检测和Southern分子杂交检测进一步表明,目的基因已整合入非洲菊基因组中。Calli were induced in vitro from the base part of the leaf petiole of C.erbara and hlended and cut into small cell masses about 300 -500 μm in diameters. Such cell masses were used as explants and plant acceptors in GUS transfnrmation mediated by Agrobacteri,tm tumefaciens. Histochemieal analysis indicated that the rate of transformed callus was more than 80%. PCR （polymerase chain reaction） and Southern hybridization analyses of eight transgenie plants showed that the interest genes had been successfully inserted into the genome of Gerbera.

关 键 词：非洲菊 小细胞团 β-葡糖酸苷酶(GUS) 基因转化 

分 类 号：Q781[生物学—分子生物学]