枸杞多糖对中波紫外线致人皮肤成纤维细胞损伤的保护作用  

Protective Effects of Lycium Barbarum Polysaccharides on Fibroblast Damaged by Ultraviolet B

作　　者：王发选[1] 宋琦如[1] 杨正贵[2] 

机构地区：[1]宁夏医学院公共卫生学院劳动卫生学教研室,银川750004 [2]宁夏医学院公共卫生学院,2005届毕业生银川750004

出　　处：《宁夏医学院学报》2006年第6期501-503,共3页Journal of Ningxia Medical College

基　　金：教育部科学技术研究重点项目(项目编号:02159)

摘　　要：目的探讨枸杞多糖(LBP)对中波紫外线(UVB)辐射致体外培养人皮肤成纤维细胞损伤的保护作用。方法采用强度30mJ/cm2的中波紫外线照射体外培养的人皮肤成纤维细胞,实验分为对照组、紫外线照射模型组和四个不同浓度LBP保护组,用MTT法检测细胞增殖活性,酶生化法检测细胞内SOD活性、MDA含量,以及培养液中LDH漏出率。结果强度为30mJ/cm2的中波紫外线可以造成体外培养的人皮肤成纤维细胞增殖活性降低,细胞内SOD活性降低,MDA含量升高,LDH漏出增加,与对照组相比均P<0.05。给定浓度范围的LBP能剂量依赖性地增加细胞的增殖活性,增加细胞内SOD活性、MDA含量,减少LDH的漏出。结论紫外线可以造成体外培养的人皮肤成纤维细胞氧化损伤,LBP对UVB致人皮肤成纤维细胞的损伤具有一定保护作用,其机制可能与它的抗氧化作用和促进细胞增殖作用有关。Objective To investigate the protective effects of Lycium Barbarum Polysaccharides（LBP） on fibroblast damaged by ultraviolet B（UVB）. Methods The fibroblast was irradiated by UVB （irradiation intensity：30mJ/cm^2） . The fibroblasts was randomly divided into six groups including 1 control group and 5 UVB radiated groups （radiated group, 0.1 mg/mL LBP, 0.2mg/mL LBP, 0.4 mg/mL LBP 0.8mg/mL LBP）. The enzymic activities were determined by MTT methods . The content of MDA 、 the activities of intracellular SOD, and the content of LDH in the supematants of cells were determined by biochemical methods. Results Compared to controls, UVB （irradiation intensity：30mJ/cm^2） can significantly decrease the proliferation activities in fibroblast and intracellular SOD and significantly increase MDA content and LDH. In the given concentration , LBP can dose-dependently significantly improve the activities of cellular proliferation and intracellular SOD and significantly decrease the contents of intracellular MDA and the content of LDH in the supematants of cells（ P 〈 0.01, respectively）. Conclusion The LBP has protective effect on fibroblast irradiated by UVB. The mechanism of the protective effect mav be associated with it＇s effect in antioxidative and enhancing celluar proliferation.

关 键 词：中波紫外线 枸杞多糖 人皮肤成纤维细胞 保护作用 

分 类 号：R285[医药卫生—中药学]