NEM对川百合花粉萌发和花粉管伸长的抑制效应  

Inhibition of N-ethylmaleimide(NEM) to Pollen Germination and Tube Growth of Lily

作　　者：张学琴[1] 陈玉玲[2] 袁明[1] 王学臣[1] 

机构地区：[1]植物生理学和生物化学国家重点实验室中国农业大学生物学院,北京100094 [2]河北师范大学生命科学院,河北石家庄050016

出　　处：《江苏农业学报》2006年第4期374-378,共5页Jiangsu Journal of Agricultural Sciences

基　　金：中国农业大学科研启动基金项目(2005026)

摘　　要：为探明微丝骨架结构以及微丝骨架对花粉萌发和花粉管伸长的作用,采用不同浓度的巯基类化学反应试剂NEM处理川百合花粉。结果表明:30μmol/L NEM能有效抑制川百合花粉萌发和花粉管伸长,抑制率分别达到95%和99%;经20μmol/L NEM处理过的川百合花粉,在去掉NEM后,其萌发率恢复到对照(0μmol/L NEM)的98%,花粉管长度恢复到对照(0μmol/L NEM)的60%;15μmol/L、20μmol/L和30μmol/L NEM处理过的川百合花粉,其花粉管内微丝排列呈弯曲状;而非沿纵轴向顶端延伸的正常微丝束状态。可见,NEM作为一种微丝骨架的抑制剂改变了花粉管中微丝骨架的分布结构,影响了花粉管的生长。Actin filament is the cytoskeleton in the pollen that controls pollen germination and tube growth. N-ethylmaleimide（NEM） is a sulfhydryla alkylating agent that can change the interaction between myosin in the cytoskeleton. To investigate the effect of NEM on actin filament and further on pollen germination and tube growth, the pollen of lily was treated with NEM at different concentrations. With the climbing of NEM concentration, actin filaments in the lily pollen become more curved, which changed the pollen from cylinder shape to zigzag curve. The inhibitions of NEM at 30 μmol/L to pollen germination and tube growth were 95% and 99%, respectively, indicating the effective inhibition of NEM. After the pollen pre-treated with NEM were put into the liquid favoring pollen germination for 80 min, germination rate and tube growth were brought back to 98% and 60% of CK（0 μmol/L NEM treatment）, respectively, suggesting that the inhibition of NEM to pollen germination and tube growth is reversible.

关 键 词：NEM 花粉萌发 花粉管伸长 微丝骨架 抑制效应 

分 类 号：Q944.42[生物学—植物学]