慢性乙型肝炎患者血清、唾液、尿液HBV-DNA水平的相关性研究  

Correlation of Hepatitis B Virus DNA between Serum、Saliva、Urine in Chronic Hepatitis B and Its Significance on Epidemiology

作　　者：潘红英[1] 谌翠容[1] 张永乐[1] 娄国强[1] 叶荣夏[1] 施军平[1] 李冰茹[1] 卢德荣[1] 

机构地区：[1]浙江省杭州市第六人民医院,310014

出　　处：《医学研究杂志》2006年第12期22-25,共4页Journal of Medical Research

基　　金：杭州市科技发展计划项目(2003133B36)

摘　　要：目的 探讨慢性乙型肝炎患者HBV-DNA在血清、唾液、尿液间可能存在的相关性及其流行病学意义。方法 采用荧光聚合酶链反应（FQ-PCR）精确定量配对检测200例慢性乙型肝炎患者血清、唾液、尿液中HBV-DNA含量。结果 200例慢性乙型肝炎患者中血清、唾液、尿液HBV-DNA检测阳性者分别为180例（90．0％）、14．5例（72．5％）、84例（42．0％）。HBV-DNA平均水平分别为血清6．51（2．69-10．00）log copies／ml，唾液4．52（2．69-7．54）logcopies／ml，尿液3．26（2．69-5．96）logcopies／ml。血清HBV-DNA与唾液、尿液HBV-DNA水平间均呈显著正相关（r分别为0．73，0．48，P〈0．001），唾液与尿液HBV-DNA之间也显著相关（r=0．73，P〈0．01）。结论 慢性乙型肝炎患者血液、唾液、尿液中含有感染性的高载量HBV-DNA，可成为传染源之一。血液中HBV-DNA含量与唾液、尿液中HBV-DNA含量有显著相关性，为慢性乙型肝炎的流行病学研究提供了新的思路和理念。Objective To explore if there are correlation between HBV - DNA levels in serum,saliva.urine in patients with chronic hepatitis B （CHB）and its significance on epidemiology. Methods Fluorescence quantitative PCR （FQ -PCR ） , which combines PCR with fluorescence probe hybridization ,was used to precisely determine HBV -DNA in serum, saliva, urine in 200 patients with CHB. Results HBV - DNA （ ＋ ） rates of serum, salvia, and urine in 200 cases of CHB patients were 90% ,72. 5% , and 42% , respectively. Average HBV - DNA contents in serum, saliva and urine were 6. 51 （ 2. 69 - 10. 00 ） , 4. 52 （ 2. 69 -7.54 ） , and 3.26 （ 2. 69 - 5.96 ） log copies/ml. Strong positive correlations of HBV - DNA levels existed in between serum and saliva, urine （r=0. 73,0. 48 ; P〈 0. 001 ） ;saliva and urine （ r= 0. 73,P 〈0. 01 ）. Conclusions Heavier loads of infectious HBV - DNA in serum,saliva,and urine have been found in CHB patients. This HBV - DNA could be one of the resources for hepatitis B infection. Positive correlation of HBV - DNA levels between serum, saliva, and urine may provide a new concept for the study of HBV epidemiology.

关 键 词：慢性肝炎 乙型 HBV-DNA 血清 唾液 尿液 流行病学 

分 类 号：R512.62[医药卫生—内科学]