丹参总酚酸抑制大鼠肾间质纤维化的初步观察  

Total salvianolic acid inhibiting renal interstitial fibrosis in rats

作　　者：尚雁君[1] 黄才国[1] 蒋山好[2] 朱大元[2] 魏善建[1] 焦炳华[1] 李锴男[3] 张建荣[4] 

机构地区：[1]第二军医大学基础医学部生物化学和分子生物学教研室,上海200433 [2]中国科学院上海药物研究所,上海201203 [3]第四军医大学基础医学部病理学教研室,西安710033 [4]第二军医大学长海医院实验诊断科,上海200433

出　　处：《第二军医大学学报》2006年第12期1295-1298,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(29632050)~~

摘　　要：目的观察丹参总酚酸对单侧输尿管梗阻(UUO)大鼠肾间质纤维化的影响。方法将40只健康、雄性SD大鼠随机分为4组分别为UUO组、假手术组(SOR)、阳性对照依那普利(ACEI)组和丹参总酚酸样品(TSA)组,术前分别给UUO组和SOR组灌生理盐水,ACEI组和丹参总酚酸组分别灌胃ACEI[6mg/(kg·d)]和丹参总酚酸[20mg/(kg·d)],连续8d。术后第7天分别处死各组大鼠用免疫组织化学方法测定TGF-β1的表达情况。行H-E和Masson染色,动态观察肾间质病理学改变。结果丹参总酚酸组和ACEI组TGF-β1的表达量与模型组(0.22±0.06)比较有明显的下降(P<0.05),结果分别是1.38±0.26和1.38±0.26。丹参总酚酸能显著减少UUO大鼠肾小管间质TGF-β1的表达,减轻胶原在肾间质的沉积,改善了肾脏病理改变。结论丹参总酚酸对UUO所致的大鼠肾脏纤维化的形成有明显的抑制作用,其作用可能跟降低TGF-β1的表达有关。Objective：To study the influence of total salvianolic acid（TSA） on the renal interstitial fibrosis caused by unilateral ureteral obstruction （UUO） in rats. Methods： Forty healthy Sprague-Dawley rats were randomly divided into 4 groups：the UUO group, sham-operation group, angiotensin-converting enzyme inhibitor （ACEI）-treated group （positive control）, and TSA-treated group. The rats in UUO group and sham-operation group received gastric gavage with normal saline for 8 days before operation; rats in ACEI-treated group and TSA-treated group received ACEI （6 mg/kg by daily gastric gavage for 8 days） and TSA （20 mg/kg by daily gastric gavage for 8 days）, respectively. All rats were killed 7 days after operation and the expression of transforming growth factor-β1 （TGF-β1） was detected by immunohistochemical method. The dynamic histological changes of renal interstitial tissues were observed by H-E and Masson staining. Results： TGF-β1 expression in TSA-treated （1.38± 0. 26） and ACEI-treated （1.38±0. 26） groups was significantly lower than that in UUO group（P〈0. 05）. TSA obviously reduced TGF-β1 expression and collagen deposition in the renal interstitial tissues and improved the renal pathological changes in UUO rats. Conclusion： TSA can evidently inhibit UUO-induced renal interstitial fibrosis in rats, which might be related with the down regulation of TGF-β1expression.

关 键 词：丹参总酚酸 肾间质纤维化 转化生长因子Β1 

分 类 号：R692[医药卫生—泌尿科学]