小鼠Wnt-3a基因在NIH3T3细胞中的表达及其活性的初步分析  

Expression and biological activity of mouse Wnt-3a gene in NIH3T3 cells

作　　者：尹宗生[1] 张辉[1] 张胜权[2] 王伟[1] 华兴一[1] 胡勇[1] 李光武[3] 

机构地区：[1]安徽医科大学第一附属医院骨科,安徽合肥230032 [2]安徽医科大学分子生物学教研室,安徽合肥230032 [3]安徽医科大学神经生物研究所,安徽合肥230032

出　　处：《第四军医大学学报》2006年第24期2212-2215,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30571881);安徽省自然科学基金(050430713)

摘　　要：目的:表达具备生物活性的重组小鼠Wnt-3a信号蛋白.方法:应用脂质体转染试剂将重组真核表达载体pSecTag2/HygroB-Wnt3a转染并筛选稳定表达的NIH3T3细胞,WesternBlot鉴定重组Wnt-3a蛋白的表达,并对Wnt3a/NIH3T3细胞的融合密度及抗凋亡能力给予检测.结果:Wnt-3a信号蛋白在Wnt3a/NIH3T3细胞中获得稳定表达,Wnt-3a信号蛋白能够明显提高NIH3T3细胞的融合密度及抗凋亡能力.结论:在NIH3T3细胞中表达的重组Wnt-3a信号蛋白具备生物活性.AIM： To express recombinant mouse Wnt-3a signal protein with biological activity. METHODS： The recombinant eukaryotic expression plasmid, pSecTag2/Hygro B-Wnt3a, was transfected into NIH3T3 cells by liposome, then the expressed protein was detected by Western Blot. The saturation density and the capability of suppressing apoptosis in Wnt3a/NIH3T3 cells were evaluated. RESULTS： The Wnt-3a signal protein was stably expressed in Wnt3a/NIH3T3 cells. The saturation density and the capability of suppressing apoptosis in Wnt3a/NIH3T3 cells were markedly increased. CONCLOUSION： We successfully construct the recombinant Wnt-3a signal protein with biological activity.

关 键 词：Wnt-3a 真核表达 接触抑制 细胞凋亡 

分 类 号：Q78[生物学—分子生物学]