异丙酚对兔肝缺血再灌注损伤保护作用及部分生化指标的影响  

Protective role of propofol against hepatic ischemia-reperfusion injury and its effect on some biochemical indicators in rabbits

作　　者：罗遥[1] 周党军[2] 吕毅[2] 景桂霞[1] 肖颖[1] 

机构地区：[1]西安交通大学医学院第一附属医院麻醉科,陕西西安710061 [2]西安交通大学医学院第一附属医院肝胆外科,陕西西安710061

出　　处：《第四军医大学学报》2006年第24期2223-2225,共3页Journal of the Fourth Military Medical University

基　　金：陕西省科学技术研究发展计划项目(2004K17-G28)

摘　　要：目的:探讨麻醉药异丙酚对兔在体肝脏缺血再灌注损伤(HIRI)保护作用及部分生化指标的影响.方法:40只家兔随机分为四组,每组10只,分别为缺血再灌注组(A组),缺血再灌注加生理盐水2mL/(kg·h)组(B组),缺血再灌注加异丙酚8mg/(kg·h)组(C组)和假手术对照组(D组).各组于再灌注后30min及60min时测定肝组织中琥珀酸脱氢酶(SDH)活性、ATP含量以及血清谷丙转氨酶(ALT),谷草转氨酶(AST)的含量.结果:再灌注后30min及60min时,A,B两组肝组织中SDH活性与D组比较明显降低(P<0.05),C组与D组比较无统计学差异(P>0.05);A,B,C组肝组织中ATP含量与D组比较均降低(P<0.05),但C组的降低程度小于同时间点的A,B两组(P<0.05);A,B,C组血清ALT,AST水平均高于D组(P<0.01,P<0.05).结论:异丙酚能增强缺血再灌注时肝细胞线粒体中SDH的活力,增加肝组织中ATP的含量,对HIRI有保护作用.AIM： To investigate the protective role of propofol against hepatic ischemia-reperfusion injury （HIRI） and the effect of propofol on some biochemical indicators in rabbits in vivo. METHODS： Forty rabbits were randomly divided into 4 groups with 10 animals each. They were HIRI model group （group A）, HIRI plus 2 mL/（ kg · h） normal sodium group（ group B）, HIRI plus 8 mg/（kg · h） propofol group （group C）, and sham operation group （group D ）. The activity of succinodehydrogenase （SDH）, ATP content in hepatic tissue and serum alanine aminotransferase （ALT） , aspartate aminotransferase （AST） content were assayed after reperfusion for 30 min and 60 min. RESULTS： SDH activity in hepatic tissue in group A and B decreased significantly compared with that in group D （ P 〈 0.05 ） and there was no difference between group C and D （ P 〉 0.05 ） after reperfusion for 30 min and 60 min; ATP content in hepatic tissue decreased in group A, B, C compared with group D （ P 〈 0.05）, but the decreased degree of group C was much smaller than group A and B at the same time （ P 〈 0.05 ） ; serum ALT, AST levels increased in group A, B, C compared with group D （P 〈 0.01, P 〈 0.05 ）. CONCLUSION ： Propofol can enhance the activity of SDH in mitochondria during ischemia-reperfusion and increase the ATP content in hepatic tissue, thus showing the protective effect against HIRI.

关 键 词：二异丙酚 肝 再灌注损伤 琥珀酸脱氢酶 腺苷三磷酸 

分 类 号：R656[医药卫生—外科学]