GAD67-GFP转基因小鼠脑干内向小脑皮层的GABA能投射  

GABAergic projection from brainstem to cerebellar cortex in GAD67-GFP transgenic mouse

作　　者：廖衡[1] 王智明[1] 王文[1] 陈涛[1] 武胜昔[1] 李云庆[1] 

机构地区：[1]第四军医大学基础部人体解剖学教研室暨梁銶琚脑研究中心

出　　处：《第四军医大学学报》2006年第24期2297-2299,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30370458;30470556)

摘　　要：目的:观察向小鼠小脑皮层投射的γ-氨基丁酸(GABA)能神经元在脑干内的分布.方法:四甲基罗达明(TMR)逆行追踪和免疫荧光组织化学相结合的双重标记方法,结果在激光共聚焦显微镜下观察.结果:将TMR注入小脑皮层后,TMR逆标神经元主要见于三叉神经脊束核(SP5),前庭上核(SuVe),前庭脊核(SpVe),前庭内侧核(MVe),前庭外侧核(LVe),耳蜗腹侧核(VC),耳蜗背侧核(DC),外侧上橄榄核(LSO),下橄榄主核(IOPr),下橄榄内侧核(IOM),下橄榄背侧核(IOD);GFP阳性神经元主要位于SuVe,MVe,LVe,DC,LSO,VC,SP5,SpVe,IOPr,小细胞网状核(PCRt),巨细胞网状核(Gi),在IOM和IOD内也能见到一些GFP阳性神经元;TMR/GFP双标神经元主要位于SuVe,MVe,LVe,DC,VC,SP5,SpVe,IOPr,在LSO,IOM和IOD内也能见到一些TMR/GFP双标神经元.结论:向小脑皮层投射的GABA能神经元在脑干内的分布比较广泛,它们可能参与了小脑对运动的调控.AIM： To observe the distribution of γ-aminobutyric acid （GABA）-containing neurons projecting to cerebellar cortex from the brainstem of the mouse. METHODS： Double-labeling method using tetramethyl-rhodamine （TMR） retrograde tracing combined with green fluorescent protein （GFP） immunohistochemical staining was utilized to observe the neurons under the laser scanning confocal microscope. RESULTS： TMR retrogradely labeled neurons were mainly found in nucleus of spinal tract of tfigeminal nerve （SP5）, superior vestibular nucleus （SuVe）, spinal vestibular nucleus （SpVe）, medial vestibular nucleus （ MVe ） , lateral vestibular nucleus （ LVe ） , ventral cochlear nucleus （VC）, dorsal cochlear nucleus （DC）, lateral superior olivary nucleus （ LSO）, principal nucleus of inferior olive （ IOPr）, medial nucleus of inferior olive （IOM） and dorsal nucleus of inferior olive （ IOD ）. GFP-like immunoreactive （ LI ） neurons were mainly observed in SuVe, MVe, LVe, DC, LSO, VC, SP5, SpVe, IOPr, parvocellular reticular nucleus （PCRt） and gigantocellular reticular nucleus （Gi）. A few GFP-LI neurons were also found in the IOM and IOD. TMR/GFP double-labeled neurons were mainly located in SuVe, MVe, LVe, DC, VC, SP5, SpVe and IOPr. Some TMR/GFP double-labeled neurons were also observed in IOM and IOD. CONCLUSION： GABA ergic neurons projecting to the cerebellar cortex are widely located in the brainstem. These GABAergic neurons are probably involved in the coordination and integration of cerebellum for locomotion.

关 键 词：Γ-氨基丁酸 小脑皮质 脑干 免疫荧光组织化学 GAD67-GFP转基因小鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]