脱蛋白骨为支架材料体外构建的组织工程骨  

Construction of tissue engineering bone with deproteinized bone as scaffold in vitro:experiment with rabbits

作　　者：范伟[1] 安洪[1] 蒋电明[1] 曹本珍[1] 

机构地区：[1]重庆医科大学附属第一医院骨科,400016

出　　处：《中华医学杂志》2006年第47期3349-3352,共4页National Medical Journal of China

基　　金：重庆市卫生局医学科研项目基金资助项目(渝卫科教[2001]46号文件01-2-017)

摘　　要：目的观察脱蛋白骨与成骨细胞在体外复合培养情况。方法用胎兔颅骨培养的成骨细胞与兔干骺端松质骨制成的脱蛋白骨体外复合培养,通过酶消化法、倒置显微镜及扫描电镜观察其生长情况。结果成骨细胞在脱蛋白骨外表面及孔隙内表面均可贴附生长,增殖、分化良好。结论体外构建的成骨细胞与脱蛋白骨的复合体具有组织工程骨的一部分生物学特性,为进一步观察其在体内的再血管化和成骨活性,用于大段骨缺损修复奠定了实验基础。Objective To construct a tissue engineering bone with deproteinized bone （DPB） as scaffold in vitro. Methods The upper end of tibia, lower end of humerus, and cancellous bone of upper end of humerus of a New Zealand rabbit were taken out to make bars of DPB. The physical and chemical characteristics and the protein content therein were tested. The morphological characteristics were observed by light, Inverted phase contrast, and stereo microscopes. Osteohlasts （OBs） were obtained from the cranial bone of a fetal New Zealand rabbit and inoculated on the scaffold of DBP. The DBP scaffolds inoculated with OBs were cultured. Enzyme digestion method was used to observe the growth of OBs. Inverted phase microscopy was used to observe the morphology and adhesion of OBs. The growth of OBs on the DPB 1 and 7 days after culture was observed by scanning electron microscopy. Results The protein content in the DPB was trivial The OBs attached to the outside and inside surfaces and grew well. Condnsion The compound body of DPB and OBs show certain biological characteristics of tissue engineering bone, thus laying an experimental foundation for further observation of its revascularization and bone formation in vivo and for the repair of large bone defect.

关 键 词：骨代用品 成骨细胞 生物医学工程 

分 类 号：R318.08[医药卫生—生物医学工程]