干湿纺聚乳酸/壳聚糖纤维交织织物作为细胞体外培养载体的实验观察  

Study On Dry-Wet Spinning PLLA /CHS Fiber Braid as Culture Carrier In Vitro

作　　者：张秀芳[1] 华慧[1] Stephen Cheng 孙皎[2] 沈新元[2] 

机构地区：[1]东华大学材料科学与工程学院,纤维材料改性国家重点实验室,上海200051 [2]上海生物材料研究测试中心,上海200023

出　　处：《上海生物医学工程》2006年第4期205-209,共5页Shanghai Journal of Biomedical Engineering

摘　　要：在体外培养的条件下观察干湿纺聚乳酸/壳聚糖纤维交织织物与成骨细胞的相容性,探讨其作为人工胸壁支架材料和人工骨支架材料的可行性。将hFOB1.19人SV40转染的成骨细胞与干湿纺聚乳酸/壳聚糖纤维交织织物体外联合培养。用扫描电镜对体外联合培养早期细胞的形态学进行观察。结果表明,成骨细胞与干湿纺聚乳酸/壳聚糖交织织物间黏附良好,具有良好的相容性,干湿纺聚乳酸/壳聚糖纤维交织织物有可能成为一种理想的可用于修复胸壁缺损和骨缺损的成骨细胞载体。To study compatibility between dry - wet spinning PLLA/CHS braid and osteoblast in - vitro and explore the possibility to reconstruct chest wall with the artificial materials. The osteoblast was cultured with the dry- wet spinning PLLA/CHS braid in vitro. The morphology of osteoblast in the early period were observed by Scanning Electron Microscope （SEM）.The results showed that osteoblast could well adhere on the surface of PLLA/CHS braid. The compatibihty of dry - wet spinning PLLA/CHS braid with osteoblast was good. It is concluded that dry - wet spinning PLLA/ CHS braid is likely to be developed into an ideal carrier of chest wall for osteoblast.

关 键 词：干湿纺 聚乳酸纤维 壳聚糖纤维 成骨细胞 人工胸壁 织物 

分 类 号：R318.08[医药卫生—生物医学工程]