α粒子和NNK联合作用的细胞毒性研究  

Study on cytotoxicities induced by alpha particle irradiation combined with NNK treatment

作　　者：李平[1] 杨陟华[1] 潘秀颉[1] 曹珍山[1] 米娜[1] 陈忠民[1] 刘刚[1] 魏菡[1] 李慧颖[1] 朱茂祥[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中华放射医学与防护杂志》2006年第6期583-586,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的研究α粒子和NNK联合作用的细胞毒性。方法指数生长期永生化的人支气管上皮细胞(BEP2D细胞)分为正常对照组(NC)、α粒子单纯照射组(α)、NNK染毒组(NNK)、NNK染毒(100μg/ml)后α粒子照射组(NNK+α)和α粒子照射后NNK染毒(100μg/ml)组(α+NNK)。用低密度接种细胞的克隆形成率测定细胞存活分数;用分子探针二氯荧光素双乙酸盐(DCFH-DA)和氢化乙啶(HE)检测细胞内活性氧(ROS)水平;通过测定培养液中乳酸脱氢酶(LDH)活性评价细胞膜通透性损伤。结果与相同剂量NNK或α粒子单独作用比较,α粒子和NNK联合作用BEP2D细胞的存活率明显下降,细胞内ROS水平和细胞培养液中LDH活性显著增高。扣除NNK效应后,α粒子和NNK联合作用BEP2D细胞的存活率明显低于α粒子单独照射组,而细胞内ROS水平和细胞培养液中LDH明显高于α粒子单独照射组。此外还发现α粒子照射后NNK染毒的细胞存活率明显低于NNK染毒后α粒子照射组。结论α粒子合并NNK的细胞毒作用具有协同性,且两者作用顺序不同对细胞存活率有影响。Objective To investigate cytotoxicities of alpha-particle irradiation combined with NNK treatment. Methods Exponentially growing immortalized human bronchial epithelial cells were divided into normal control group （NC）, alpha particle irradiation group （α）, NNK administration group （NNK）, NNK administration （100 μg/ml） followed by alpha particle irradiation group （NNK ＋ α）, and alphaparticle irradiation followed by NNK administration （100 μg/ml） group （α ＋ NNK）. Cell survival fractions were measured by cloning rate of low-density plating cell. Ethidium bromide and 2＇, 7＇-dichlorofluorescein, fluorescent products of the membrane-permeable dyes hydroethine and 2＇ ,7＇-dichloroflurescindiacetate were used to monitor the intracellular reactive oxygen species （ROS）. Damage to membrane permeability was evaluated through testing LDH activity in medium. Results In the groups exposed to both alpha particles and NNK, the survival rates were significantly lower than that of the groups administrated with the same dose of alpha particles or NNK alone. The levels of intracellular ROS and the activity of LDH in medium were significantly higher than that of the groups administrated with the same dose of alpha particles or NNK alone. Subtracted the NNK effect, the survival rates of the groups received both alpha particle irradiation and NNK treatment were significantly lower than that of alpha particle irradiated only group. However, the intracellular ROS level and the activity of LDH in medium were significantly higher than that of alpha-particle irradiated only group. In addition, the survival rates of the cells in groups exposed to alpha particle irradiation followed by NNK administration were significantly lower than that of cells treated with NNK administration followed by alpha particle irradiation. Conclusions Alpha particle irradiation and NNK administration had synergisticity in cytotoxicity, and furthermore different schedules of the administration resulted in different su

关 键 词：α粒子照射 NNK BEP2D细胞 细胞存活率 活性氧 细胞膜损伤 

分 类 号：R114[医药卫生—卫生毒理学]