洛美沙星-茜素-镧三元体系光度测定洛美沙星胶囊含量  

UV-SPECTROPHOTOMETRIC DETERMINATION OF LOMEFLOXACIN CONTENT IN DRUG CAPSULES BY THE TERNARY REACTION SYSTEM AMONG LOMEFLOXACIN,ALIZARIN AND LANTHANUM

作　　者：祝优珍[1] 严怡君[1] 徐莉英[1] 

机构地区：[1]上海应用技术学院化学工程系,上海200235

出　　处：《理化检验（化学分册）》2006年第12期1034-1036,1068,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：上海市教委自然科学基金资助课题(No.03HK13)

摘　　要：应用洛美沙星（LMX）-茜素-镧形成的三元化合物，在紫外光区有明显的吸收，最大吸收波长为286nm，在0．7～12mg·L^-1范围内服从比耳定律。其摩尔吸光系数为4．2×10^4L·mol^-1·cm^-1。应用此方法分析了洛美沙星胶囊中LMX的含量，所得结果与文献[15]方法的结果一致。测定结果的平均RSD值为0．95％，回收率的平均值为101．1％。加入反应物的次序对测定结果有影响，必须按照先加洛美沙星，其次茜素，最后加镧的顺序。反应物的最佳摩尔比为1：1；2（洛美沙星：茜素：镧），以氯仿作为溶剂。反应的温度和时间对测定结果影响不明显。反应产物在2h内稳定。Based on the absorption behavior of the ternary complex formed among lomefloxacin, alizarin and lanthanum in the ultra-violet band with its absorption maxima at 286 urn, an UV-spectrophotometric method for the determination of lomefloxacin was proposed. Linear relationship between the absorbance and concentration of lemofloxacin was found in the range of 0.7-12 mg · L^- 1, with molar absorptivity of 4. 2× 10^4L · mol^-1 · cm^-1. As shown by experimental results, the addition of reagents should be in the order of adding firstly alizarin solution to the sample solution containing lomefloxacin, and then adding the La（ Ⅲ ） solution. The mole ratio of the ternary complex was found to be [lomefloxacin ： alizarin ： La（ Ⅲ）] 1 to 1 to 2. Chloroform was used as solvent to remove excess of alizarin by extraction. The ternary complex was stable within 2 h. Temperatures within the range of 20- 70 ℃ were tolerable for the eomplexing reaction. In the analysis of a sample of lomefloxacin capsules, the results obtained by this method were found in consistency with the labelled value and also with the results obtained by the method given in literature^[15] , Average values of RSD＇s was 0. 95% and of recovery was 101.1%.

关 键 词：紫外分光光度法 洛美沙星 茜素 镧(Ⅲ) 微量分析 

分 类 号：O657.3[理学—分析化学]