酸性橙Ⅱ与牛血清白蛋白相互作用的电化学研究  

Electrochemical study on interaction of acid orange Ⅱ with bovine serum albumin

作　　者：李有琴[1] 郭玉晶[1] 潘景浩[1] 

机构地区：[1]山西大学化学化工学院

出　　处：《分析试验室》2006年第12期35-38,共4页Chinese Journal of Analysis Laboratory

基　　金：山西省自然科学基金(20041015)项目资助

摘　　要：以电化学法和光度法对酸性橙Ⅱ（AOⅡ）与牛血清白蛋白（BSA）的相互作用进行了研究。在pH7．20的Tris—HCl缓冲溶液中，AOⅡ有一灵敏的还原峰，峰电位Ep约为-0．69V（vs.SCE），加入BSA后AOⅡ还原峰电位正移，峰电流下降，据此建立了一种测定BSA的电分析方法。在优化条件下，峰电流下降值与BsA的浓度在5．0×10^-8 -1．0×10^-5 mol／L（r=0．9900-0．9939）范围内呈良好的线性关系，检出限为3．0×10^- 8mol／L。测定了AOⅡ与BSA的结合比和结合常数，并对结合反应机理进行了初步的探讨。The interaction of acid orange Ⅱ with BSA was studied by electrochemical method and spectrophotometry. In pH 7.20 riffs - HCl buffer, AO Ⅱ has a sensitive reduction peak at - 0.69 V （vs. SCE）. The addition of BSA to the above solution, the reduction peak current of AO Ⅱ decreases and reduction peak potential shifts positively. An electrochemical quantitive method of BSA was developed based on the interaction of AO Ⅱ with BSA. Under the optimized condition, the decrease of the reduction peak current of AO Ⅱ is proportional to the concentration of BSA in the range of 5.0 × 10^-8- 1.0 × 10^-5 mol/L. The limit of detection is 3.0 × 10^-8 mol/L. The binding ratio and binding constant of DNA with BBG were calculated. Furthermore, the binding mechanism was also preliminarily discussed.

关 键 词：酸性橙H 牛血清白蛋白 相互作用 

分 类 号：O657.1[理学—分析化学]