单铽血清转铁蛋白与单铽伴清蛋白铽(Ⅲ)脱除的荧光动力学比较  

The Comparation of Terbium(Ⅲ) Removal from Monoterbium Serum Transferrins and Monoterbium Ovotransferrins by Fluorescence Spectra

作　　者：白海静[1] 崔文平[2] 杨斌盛[1] 

机构地区：[1]山西大学分子科学研究所,太原市030006 [2]太原铁路机械学校,太原市030006

出　　处：《光谱实验室》2006年第6期1274-1277,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：山西省自然科学基金资助课题(No.20031017)

摘　　要：在0.01m ol.L-1N-2-羟乙基哌嗪-N′-2-乙磺酸(H epes),pH 7.4,室温条件下,用荧光法研究酒石酸脱除单铽转铁蛋白中铽(Ⅲ)的动力学、各种阴离子对单铽血清转铁蛋白脱除的影响及伴清蛋白阴离子结合部位。结果表明:单铽血清转铁蛋白与单铽伴清蛋白的Tb(Ⅲ)脱除在衰减趋势、曲线拟合及表观速率常数与脱除剂浓度的关系上表现出明显差异,伴清蛋白的脱除动力学更为复杂莫测。In 0.01mol·L^-1 N-（2-hydroxyethyl）-piperazine-N′-2-ethanesulfonic acid（Hopes）at room temperature and pH7.4,the removal behavior of Tb（ Ⅲ ） from monoterbium-ovotransferrins by tartaric acid was studied by fluorescence spetra. The Tb （ Ⅲ ） removal pathway is the complex with saturation and first order for TbcTf,while it is a simple first order pathway for TbNTf. The removal curves of Tb （ Ⅲ ） from TbNOTf are difficult in the curve-fitting by simple mono-exponential equation. Although the removal curves of Tb （ Ⅲ ） from TbcOTf have a good fitness,kobs descends to a certain point with the increase of the concentration of removal ligand.

关 键 词：单铽转铁蛋白 荧光光谱 动力学 阴离子 

分 类 号：O657.32[理学—分析化学]