链霉素对心肌梗死大鼠离体心脏牵张时心律失常的影响  

The effect of streptomycin on arrhythmia caused by stretch of isolated myocardial infarcted hearts in rats.

作　　者：富路[1] 曹君娴[1] 谢荣盛[1] 

机构地区：[1]哈尔滨医科大学附属第一医院心内三科,黑龙江哈尔滨150001

出　　处：《中国心脏起搏与心电生理杂志》2006年第6期515-518,共4页Chinese Journal of Cardiac Pacing and Electrophysiology

摘　　要：目的探讨链霉素对心肌梗死(简称MI)大鼠离体心脏牵张时心律失常的影响。方法36只wistar大鼠随机分为正常对照组、链霉素组、MI组、MI+链霉素组。离体心脏经Langendorff灌流后,通过改变水囊容积,以△V=0.1,0.2,0.3ml对心室牵张5s,观察牵张效应30s,记录90%单相动作电位时程(MAPD90)、室性早搏(PVB)及室性心动过速(VT)的次数。结果牵张使正常对照组及MI组MAPD90显著延长(P<0.05或0.01),其中MI组在相应牵张幅度下其MAPD90增加更为显著(与正常对照组比较,P<0.01;如△V=0.3ml,从63.2±4.95ms到55.67±4.39ms)。链霉素对基础状态下MAPD90无影响(P>0.05),但可使牵张后已延长的MAPD90显著缩短(P均<0.05;如MI组与MI+链霉素组,△V=0.2ml,从58.09±4.27ms到53.1±3.64ms)。正常及MI组PVB和VT发生率随△V的增加而增加,且后者高于前者。链霉素使两组PVB发生率显著下降,并可显著抑制VT的发生(P<0.005)。结论MI后牵张更有助于恶性心律失常的产生和维持,链霉素可以明显抑制上述现象的发生。Objective To study the effect of streptomycin on arrhythmia caused by stretch of isolated myocardial infracted（MI） hearts in rats. Method 36 wistars were randomly divided into 4 groups： normal control group, streptomycin group ,MI group,MI ＋ streptomycin group. After perfused on Langendorff, the hearts were stretched for 5 s by 0.1,0.2 and 0.3 ml volume change respectively. The effect of stretching was observed for 30 s, including 90% monophasic action potential（MAPD90） ,premature ventricular beats（PVB） and ventricular tachycardia（VT）. Results MAPDgo in normal control and MI group obviously lengthened after the hearts were stretched（ P 〈 0.05 or 0.01 ）. And MAPDgo in MI group was even longer than that of normal control group （ P 〈 0.01 ; for example ： A V = 0.3 ml, from 63.2 ± 4.95 ms to 55.67 ± 4. 39 ms）. Streptomycin had no influence on MAPD90 of basic state （ P 〉 0.05 ）, while it could reduce the lengthened MAPD90 after stretched（P 〈 0.05 ; for example：When MI group compared with MI ＋ streptomycin group, AV =0.2 ml, from 58.09 ±4.27 ms to 53.1 ± 3.64 ms）. The incidence rate of PVB and VT in normal control and MI group increased as AV increased. And the incidence rate of PVB and VT in MI group was higher than that in normal control group. 200 μmol/L streptomycin could reduce the incidence rate of PVB, and obviously inhibit the occurrence of VT（P 〈 0. 005 ）. Conclusion MI could initiate the generation and maintenance of malignant arrhythmia. Streptomycin could obviously inhibit the occurrence of malignant arrhythmia.

关 键 词：心血管病学 链霉素 牵张激活非选择性离子通道 机电反馈 单向动作电位 

分 类 号：R331.38[医药卫生—人体生理学] R978.12[医药卫生—基础医学]