糖尿病状态对甲钴胺预防大鼠外周神经病变作用的影响  

Effect of Diabetic Status on the Preventive Efficiency of Methylcobal on Peripheral Neuropathy in Rats

作　　者：李剑波[1] 陈家伟[1] 李晓璐[2] 

机构地区：[1]南京医科大学第一附属医院内分泌科,江苏南京市210029 [2]南京医科大学第一附属医院电神经生理室,江苏南京市210029

出　　处：《中国康复理论与实践》2006年第10期861-862,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：江苏省科委基金部分资助(No.BJ99061)

摘　　要：目的 探讨血糖水平和病程对甲钴胺预防糖尿病外周神经病变（PN）作用的影响。方法 80只SD大鼠随机选取16只为正常对照组，余64只制作四氧嘧啶诱导糖尿病模型，后者又根据外源胰岛素控制血糖水平分为血糖控制较好组和较差组各32只，每组再选取16只给予甲钴胺（500μg／kg）肌注。分别在造模前和造模后2周、8周、12周测定大鼠的坐骨神经运动、感觉和相应诱发电位的波幅，进行果糖胺等糖代谢监测。结果 注射甲钴胺的血糖控制较好组大鼠（果糖胺1.0mmol／L）运动、感觉神经传导速度和波幅的下降比血糖控制较差组（果糖胺1．2mmol／L）明显延缓（P〈0．05～0．01）。至第8周，注射甲钴胺的血糖控制较好组大鼠的电生理指标与正常对照组无显著性差异；12周时，血糖控制较差组大鼠中，注射甲钴胺大鼠的电生理指标与未注射甲钴胺的无显著性差异（P〉0．05）。未见甲钴胺对血糖的影响。结论 甲钴胺延缓糖尿病PN的效应受血糖和病程的影响，病程早期、血糖控制较者效果较好。Objective To investigate the effect of varied hyperglycemia and durations on preventing peripheral neuropathy （PN） with methylcobal in diabetic rats. Methods 80 Sprague Dawley rats were selected and randomly chosen 16 as normal control （NC） group, others 64 animals as diabetic model induced by alloxan. The model rats were evenly divided into relatively good control （GC） group and relatively poor control （PC） group based on levels of hyperglycemia regulated with exogenous insulin. 16 rats of each GC/ PC group were intramuscularly injected with methylcobal （500μg/kg）. The sensory nerve conduction velocity （SNCV）, motor nerve conduction velocity （MNCV） and evoked potential amplitute （EPA） of sciatic nerve of rats were detected by evoked electromyogram respectively at 2nd, 8th and 12th week after onset of diabetes. Fructosamine in serum was also detected. Results The rats injected with methylcobal had a significantly delayed reduction of SNCV, MNCV and EP in GC group （fructosamlne, 1.0 mmol/L） than that in PC group （fructosamine, 1.2 mmol/L） （ P 〈0.05-0.01）. Up to 8th week after onset of diabetes, there was no obvious difference in physioelectroparameters between the GC group and NC group. At 12th week after onset of diabetes, physioelectroparameters in the PC group fell to the level of non-methylcobal injected group. No effect of methylcobal on blood glucose revealed in any groups （ P〉0.05）. Conclusion Methylcobal has a beneficial effect on delaying the onset of diabetic PN, and is of high efficacy with a good control of diabetic state, especially at early stage.

关 键 词：糖尿病 甲钴胺 外周神经病变 大鼠 

分 类 号：R587.1[医药卫生—内分泌]