青花菜细胞质雄性不育系线粒体DNA的提取与RAPD分析  

Extraction and RAPD Analysis of Mitochondrial DNA of Cytoplasmic Male Sterile(CMS) Line in Broccoli

作　　者：朱玉英[1] 杨晓锋[1] 侯瑞贤[1] 李式军[2] 吴琴生[2] 

机构地区：[1]上海市农业科学院园艺研究所 [2]南京农业大学园艺学院,南京210095

出　　处：《武汉植物学研究》2006年第6期505-508,共4页Journal of Wuhan Botanical Research

基　　金：上海市科技兴农重点攻关项目[农科攻字(96)第5-02号]

摘　　要：利用分子生物学方法提取青花菜不育系及其保持系线粒体的DNA,经琼脂糖凝胶电泳表明所提取的线粒体DNA纯度较高。通过40个引物进行随机引物多态性扩增,结果表明,由引物S66扩增出一条1 000 bp左右的特异条带BMS1000,该条带是不育系m tDNA所特有的,初步推断该特异条带可能与不育性相关。DNA from the mitochondrion （mtDNA） of CMS line BC7-19 and its maintaner of broccoli （Brassica oleracea L. var. italica Plenck. ） was extracted by molecular bio-technique, and was marked using RAPD reaction. The result showed that the purity of the extracted mtDNA was high by sepharose electrophoresis. And the result also showed that a special fragment,BMS1000 ,with about 1 000 bp amplificated by primer $66, was peculiar for the mtDNA of male sterile line by RAPD reaction of 40 primers, and the special fragment was initially considered to be relevant to male sterility after sequence analysis.

关 键 词：青花菜 雄性不育系 线粒体DNA RAPD 

分 类 号：S603.2[农业科学—园艺学] Q949.748.3[生物学—植物学]