产前应激子代大鼠海马核转录因子-κB表达的性别差异  

Gender-dependent difference of NF-κB expression in the hippocampus of prenatally stressed offspring rats

作　　者：李晖[1] 朱忠良[2] 贾宁[2] 白转丽[2] 蔡青[2] 陈蕊[3] 宋天保[1] 刘健康[4] 

机构地区：[1]西安交通大学医学院人体解剖学与组织胚胎学系 [2]西安交通大学医学院生理学与病理生理学系,西安710061 [3]西安交通大学医学院第一附属医院儿科,西安710061 [4]中国科学院上海生命科学研究院营养研究所

出　　处：《生理学报》2006年第6期577-583,共7页Acta Physiologica Sinica

基　　金：This work was supported by the National Natural Science Foundation of China (No. 30670672).

摘　　要：本研究采用免疫组织化学和Westernblot检测NF-κBp65/p50在产前应激子代海马的表达,并探讨其表达是否存在性别差异。研究结果显示,在雌性子代,中、晚期应激组海马齿状回p65表达显著低于对照组(P<0.01),而海马各区p50表达均显著高于对照组(P<0.01),中、晚期应激组间p65和p50表达均有显著差异(P<0.01)。在雄性子代,中、晚期应激组海马齿状回p65表达显著高于对照组(P<0.01),晚期应激组海马各区p50表达均显著低于对照组(P<0.05,P<0.01),中、晚期应激组间p65和p50表达均有显著差异(P<0.01)。雌、雄子代比较,对照组雌、雄p65表达差异极显著(P<0.01),p50仅在海马CA1区表达差异极显著(P<0.01);中期应激组雌、雄子代大鼠海马p65/p50表达无显著差异;晚期应激组雌、雄海马p65/p50表达均有极显著差异(P<0.01)。Westernblot与免疫组织化学结果基本一致。结果表明,产前不同时期的应激显著影响子代海马NF-κBp65和p50表达,且有性别差异,这可能是产前应激对子代雌、雄大鼠学习记忆能力影响差异的机制之一。In this study, immunohistochemistry and Western blot were used to determine whether the expression of NF-κB in the hippoeampus of prenatally stressed offspring rats is gender-dependent. The results were as follows： In the female offspring rats, the expressions of D65 in the hippoeampal dentate gyms in mid-term stress （MS） and late-term stress （LS） groups were significantly less than that in the control group （P〈0.01）. There was a significant difference between MS and LS groups （P〈0.01）. The expressions of p50 in all regions of hippoeampus in MS and LS groups were significantly more than that in the control group （P〈0.01）. A significant difference was also present between MS and LS groups （P〈0.01）. In the male offspring rats, the expressions of D65 in the hippoeampal dentate gyrus in MS and LS groups were evidently more than that in the control group （P〈0.01）. There was a significant difference between MS and LS groups （P〈0.01）. The expressions of p50 in all regions of hippocampus in MS and LS groups were significantly less than that in the control group （P〈0.05, P〈0.01）. There was also a significant difference in p65 expression between MS and LS groups （P〈0.01）. In addition, in the control group the expressions of p65 in the hippoeampal dentate gyms of female offspring rats were significantly more than that of male ones （P〈0.01）. However, in LS group the expressions of p65 in the hippoeampal dentate gyms of female offspring rats were significantly less than that of male ones （P〈0.01）. Moreover, there was no significant difference in p65 expression between female and male offspring rats in MS group. In the control group the gender difference in the expression of p50 was only observed in hippocampal CA1 （P〈0.01）. The expressions of p50 in all regions of hippocampus of female offspring rats were significantly more than that of male ones in LS group （P〈0.01）. There was no significant difference in p50 expression between female and ma

关 键 词：核转录因子-ΚB 产前应激 性别差异 海马 

分 类 号：R714[医药卫生—妇产科学]