维生素C多聚磷酸酯对小鼠肝脏抗氧化物酶基因转录的影响  

Effects of vitamin C polyphosphate on antioxidase gene transcription activity in mouse liver

作　　者：滕旭[1] 武文琦[2] 周显青[3] 

机构地区：[1]首都医科大学生化与分子生物学系,北京100069 [2]首都医科大学蛋白质组学平台,北京100069 [3]首都医科大学基础医学院实验动物学系,北京100069

出　　处：《动物学报》2006年第6期1107-1112,共6页ACTA ZOOLOGICA SINICA

基　　金：北京市教育委员会课题资助(2002KJ085)~~

摘　　要：为研究维生素C多聚磷酸酯对小鼠肝脏脂质抗氧化物酶基因转录的影响,将24只3-4周龄、体重为16-22g的健康雄性小鼠随机分为4组,分别在饵料中添加0、500、2500和5000mg/kg的35%维生素C多聚磷酸酯,喂食4周后取其肝脏,用Trizol法抽提总RNA,利用RT-PCR方法对小鼠肝脏超氧化物歧化酶基因、过氧化氢酶基因和谷胱甘肽过氧化物酶基因的mRNA进行分析。结果表明,维生素C多聚磷酸酯对小鼠肝脏抗氧化物酶基因的转录有显著性影响(P<0·05)。维生素C多聚磷酸酯添加量为2500和5000mg/kg的两组,过氧化氢酶基因的mRNA水平明显高于对照组;维生素C多聚磷酸酯添加量为2500和5000mg/kg的两组超氧化物歧化酶基因的mRNA水平明显高于对照组,其中5000mg/kg组的mRNA水平明显高于其它三组;维生素C多聚磷酸酯添加量为5000mg/kg组,谷胱甘肽过氧化物酶基因的转录活性明显高于其它三组(P<0·05)。研究结果表明:高剂量的维生素C多聚磷酸酯能促进小鼠抗氧化物酶基因的转录活性,但促进不同抗氧化物酶基因转录所需的维生素C多聚磷酸酯的量不同。The study was conducted to investigate the effects of vitamin C polyphosphate on transcription activity of antioxidase gene in mice liver. 24 male mice （3 - 4 weeks old） with an initial body weight of 16 - 22 g were randomly divided into four groups. These were fed with 35 % vitamin C polyphosphate supplementation at the dosages of 0, 500, 2 500 and 5 000 mg/kg of the diet, respectively for 4 weeks. In order to evaluate transcription activity of liver antioxidase gene, livers were collected to detect the mRNA of superoxide dismutases （SOD）, catalase （CAT） and glutathione peroxidase （GSH-Px） by RT-PCR. Results showed that vitamin C polyphosphate had significant effects on transcription activity of antioxidase gene in mouseliver. The CAT mRNA in the groups supplemented with vitamin C polyphosphate at 2 500 and 5 000 mg/kg were significantly higher than that of control group. The SOD mRNA in the groups supplemented with vitamin C polyphosphate at 2 500 mg/kg were higher than that of the control group, and the 5 000 mg/kg group were higher than those of other three groups. The GSH-Px mRNA in the groups supplemented with vitamin C polyphosphate at 5 000 mg/kg was notably higher than those of other three groups. These results suggest that vitamin C polyphosphate can improve the transcription activity of the antioxidase gene in mouse liver, while the requirement for vitamin C polyphosphate to improve transcription activities of the antioxidase gene varies .

关 键 词：小鼠 维生素C多聚磷酸酯 超氧化物歧化酶 过氧化氢酶 谷胱甘肽过氧化物酶 

分 类 号：Q75[生物学—分子生物学]