荧光定量PCR弓形虫DNA阳性感染者血清Th1/Th2型细胞因子的研究  

Study on variations of serum Th1/Th2 type cytokines in fluorescent quantitative PCR toxoplasma DNA positive infecters

作　　者：王雪楠[1] 陈东风[1] 何华[1] 

机构地区：[1]济宁医学院附属医院,山东济宁272029

出　　处：《中国病原生物学杂志》2006年第6期432-433,共2页Journal of Pathogen Biology

摘　　要：目的探讨荧光定量PCR(FQ-PCR)弓形虫DNA阳性感染者血清Th1/Th2型细胞因子的变化。方法用酶联免疫吸附试验(ELISA)法检测38例FQ-PCR弓形虫DNA阳性感染者血清中细胞因子IL-2和IL-6的水平,16例抗弓形虫IgG抗体阳性、FQ-PCR DNA阴性和17例抗弓形虫IgG抗体、FQ-PCR DNA双阴性的健康志愿者作为对照。结果荧光定量PCR弓形虫DNA阳性感染者血清Th1型细胞因子IL-2为(0.67±0.51)ng/ml,两对照分别为(2.97±2.86)ng/ml和(3.14±3.05)ng/ml,差异有显著性(P<0.01);Th2型细胞因子IL-6 FQ-PCR弓形虫DNA阳性感染组为(22.33±18.27)ng/ml,两对照组分别为(4.79±3.92)ng/ml和(3.26±1.13)ng/ml,差异有显著性(P<0.01)。抗弓形虫IgG抗体阳性、DNA阴性病人组Th1型细胞因子IL-2和Th2型细胞因子IL-6水平与健康对照组比较均无显著变化(P均>0.05)。结论FQ-PCR弓形虫DNA阳性感染者Th1/Th2型细胞因子水平发生了显著改变,提示FQ-PCR弓形虫DNA阳性感染者体内Th1/Th2型细胞亚群飘移。Objective To study the variations of serum Th1/Th2 type cytokines in fluorescent quantitative PCR（FQ-PCR） toxoplasma DNA positive infeeters. Methods Enzyme linked immunosorbent assay（ELISA） was used to determine the levels of Interleukin-2 （IL-2） and Interleukin 6 （IL-6） in 38 FQ-PCR toxoplasma DNA positive patients. 16 patients of antitoxoplasma IgG antibody positive but FQ PCR DNA negative and 17 volunteers of antitoxoplasma IgG antibody and FQ-PCR DNA double negative as controls. Results The level of serum Th1 type cytokine IL-2 in FQ-PCR toxoplasma DNA positive patients was （0.67 ±0.51 ） ng/ml, significantly lower than the other two control groups [（2.97 ±2.86） ng/ml and （3.14±3.05） ng/ml] , （P〈0.01） ;Level of serum Th2 type cytokine IL-6 in FQ PCR toxoplasma DNA positive patients was（22.33±18.27） ng/ml,the other two control groups were （4.79±3.92） ng/ml and（3.26± 1.13） ng/ml, the distinction were significant （P〈0.01）. The levels of Th1 type cytokine IL-2 and Th2 type cytokine IL-6 were no significant change between the antitoxoplasma IgG antibody positive but DNA negative patients and the health controls （P〉0.05）. Conclusion The serum Th1/Th2 type cytokines occured significant change in FQ-PCR toxoplasma DNA positive infecters, reminded the float between Th1 and Th2 lymphocyte subgroups.

关 键 词：荧光定量PCR 弓形虫 TH1/TH2细胞因子 IL-2 IL-6 

分 类 号：R382.5[医药卫生—医学寄生虫学]