高效液相色谱法测定珍菊降压片中绿原酸、盐酸可乐定、氢氯噻嗪的含量及含量均匀度  

Determination of clonidine hydrochloride,hydrochlorothiazide and chlorogenic acid and uniformity in Zhenju Jiangya tablets by HPLC

作　　者：鲁敏[1] 龚青[1] 金樟照[1] 戚雁飞[1] 

机构地区：[1]浙江省药品检验所,杭州310004

出　　处：《中国现代应用药学》2006年第6期495-497,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 建立高效液相色谱法测定珍菊降压片中盐酸可乐定、氢氯噻嗪的含量及含量均匀度。方法 采用C18柱（2．50mm×4．6mm，5μm），测定盐酸可乐定的流动相为乙腈-0．2％磷酸（5：95）、检测波长为210nm；测定氢氯噻嗪的流动相为甲醇-0．1％磷酸（15：85），检测波长为332nm；流速1mL／min。结果 线性范围：盐酸可乐定7．132～142．64ng（r^2=1．0000）；绿原酸0．0511～0．61321μg（r=0．9999）；氢氯噻嗪1．0023～12．02761μg（r=1．0000）。加样回收率：盐酸可乐定为99．5％，RSD为2．7％（n=9）；绿原酸为99．7％，RSD为1．57％（n=9）；氢氯噻嗪为100．4％，RSD为1．03％（n=9）。结论 本方法操作简单、结果准确，可作为控制本品质量的方法。OBJECTIVE To develop HPLC methods for determination of clonidine hydrochlofide, hydrochlorothiazide and chlorogenie acid uniformity in Zhenju Jiangya tablets. METHODS The HPLC analysis was performed on a Agilent Zorbax Extend C18 （250mm×4.6mm ,5μm）, elonidine hydrochloride was separated using the mobile phase consisted of acetanilide and 0.2% H3 PO4 （5： 95）, detected at 210nm, hydrochlorothiazide and ehlorogenie acid were done using the mobile phase consisted of methanol and 0.1% H3PO4 （15：85 ）, monitored at 332nm, flow rate was 1.0mL·min^-1. RESULTS The linearity ranges of elonidine hydrochloride, chlorogenic acid and hydrochlorothiazide were 7. 132 - 142.64ng （r^2 = 1. 000 0）, 0. 0511 -0. 61320g （r = 0. 999 9 ）, 1. 0023 - 12.0276μg（ r = 1. 000 0）, the recovery rate of analytical method was 99.5% ,RSD was 2.2% （ n =5） for elonidine hydrochloride, was 99.7% , RSD was 0.92% （ n = 6） for chlorogenic acid, was 100.4% , RSD was 1.03% （ n = 6） for hydrochlorothiazide, respectively. CONCLUSION The methods developed are simple and accurate, and can be used to control the quality of Zhenju Jiangya tablets.

关 键 词：珍菊降压片 盐酸可乐定 氢氯噻嗪 绿原酸 高效液相色谱法 

分 类 号：R917.720.1[医药卫生—药物分析学]