发酵虫草菌粉中甘露醇含量的比色法测定  

Determination of mannitol in fermented Cordyceps sinensis by colorimetric method

作　　者：杨晓东[1] 崔勤敏[2] 朱妙琴[3] 吴永江[4] 

机构地区：[1]金华职业技术学院医学院,浙江金华321007 [2]浙江医学高等专科学校,杭州310053 [3]浙江教育学院,杭州310012 [4]浙江大学现代中药研究所,杭州310058

出　　处：《中国现代应用药学》2006年第6期504-506,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 对比研究发酵虫草菌粉中甘露醇的提取和后处理方法，建立甘露醇含量的比色测定法。方法 采用超声提取、高速离心分离进行样品预处理，比色法测定发酵虫草菌粉中甘露醇的含量。结果 甘露醇的线性范围为20～90μg／mL，加样回收率为102．6％，RSD为1．34％。重复性实验的RSD为0．44％，供试品溶液在24h内稳定。2001年生产的发酵虫草菌粉中甘露醇含量的平均值比前2年约高2％，含量变化范围比前两年稍小，3年内生产的各批次甘露醇含量波动均在10％左右，发酵虫草菌粉质量较为稳定。结论 用超声离心比色法测定甘露醇含量，简捷快速，重现性好，回收率高，可以用于发酵虫草菌粉的质量控制。OBJECTIVE To select the optimal method of extracting mannitol from Cordyceps sinensis （ Berk. ） Sacc. for determining the content of mannitol in the herb using colourimetric method. METHODS Ultrasonic extraction following centrifugation was used for extracting the active compound from the herb. RESULTS The linear range of mannitol was 20 - 90μg/mL and the recovery was 102.6% with RSD 1.34% （n =5 ）. Variation coefficient was 0.44% and the sample solution was stable in 24 h. The proposed method was applied to determine the content of mannitol in 60 batches of fermented Cordyceps sinensis （ Berk. ） Sacc. produced in the years of 1999, 2000 and 2001. The average concentration of mannitol in the fermented herb produced in 2001 was about 2% higher than those in 1999, 2000 and with less remarkable variation, the content of mannitol in 60 batches of the herb produced in the 3 years was found to fluctuate at 10%. CONCLUSION The proposed method is effective and convenient for the analysis of mannitol in quality control of Cordyceps sinensis （ Berk. ） Sacc..

关 键 词：发酵虫草菌粉 甘露醇 比色法 超声提取 

分 类 号：R917.720.2[医药卫生—药物分析学]