新生大鼠中枢神经系统不同区域细胞生长的形态学比较研究  

A Comparative Morphology Study on the Growth of Cells Isolated from Different Regions of Neonatal Rat's CNS

作　　者：刘佳[1] 罗湘颖[1] 杨志敏[2] 王廷华[1] 刘苏[1] 赵匡彦[2] 

机构地区：[1]昆明医学院神经科学研究所,昆明650031 [2]昆明医学院第一附属医院神经外科,昆明650032

出　　处：《昆明医学院学报》2006年第6期27-29,T0004,共4页Journal of Kunming Medical College

摘　　要：目的分离培养新生sD大鼠的中枢神经系统不同区域细胞并观察其生长情况．方法分离出生后1～3dSD大鼠大脑皮质、小脑皮质、海马、脑室下区、脑干、脊髓细胞，加入含有10％小牛血清的DMEM／F12培养基进行培养，在倒置相差显微镜下观察其生长情况并进行对比研究，于第10天将细胞固定后采用免疫细胞化学技术检测特异性神经元抗原NSE和特异性星形胶质细胞抗原GFAP的表达．结果经观察和免疫细胞化学鉴定后发现各区域均有神经元和星形胶质细胞生长至第10天，细胞生长旺盛，神经元胞体呈三角形或椭圆形，体积较大，四周晕光明显，神经突起多而粗大，分枝互成网络；星形胶质细胞细胞核为椭圆形，常偏于胞体的一侧，胞突丰富，分支多．结论我们培养成活了新生SD大鼠中枢神经系统各区域细胞．对比研究了各区域细胞的生长情况．并鉴定了各区域中的神经元和星形胶质细胞．Objective To isolate and identify the cells from the cerebral cortex, cerebellar cortex, hippocampus, sub - ventricle region, brain stem and the spinal cord of neonatal rats and observe their growth morphology in vitro. Methods The cells were incubated from different regions of the CNS of neonatal rats by using DMEM/F12 media added with 10% bovine serum, and their growth morphology was observed by using inverted phase contrast microscope; then in the 10th day after incubation cells were fixed and immunocytochemical method was used to detect specific NSE antigen of the neurons and specific GFAP antigen of the astrocytes. Results Both neurons and astrocytes were studied in each region and they bloomed in the 10th day after incubation. Neurons had big triangular or ellipsoidal cell bodies surrounded with a halo and had robust nervous process which interlaced each other around cell bodies. The astrocytes had an ellipsoidal nucleus located at one side of the cell body and they had abundant processes branching profusely. Conclusion A method of culturing cells from different regions of the CNS of neonatal rats was described. A comparative morphology study of their growth was made and neurons and astrocytes of all the regions studied were identified.

关 键 词：细胞培养 中枢神经系统 神经元 星形胶质细胞 免疫细胞化学 分离 鉴定 

分 类 号：R651[医药卫生—外科学]