Molecular Markers for Leaf Rust Resistance Gene Lr45 in Wheat Based on AFLP Analysis  

作　　者：ZHANG Na YANG Wen-xiang YAN Hong-fei LIU Da-qun CHU Dong MENG Qing-fang ZHANG Ting 

机构地区：[1]Department of Plant Pathology, College of Plant Protection/Biological Control Center of Plant Diseases and Pests of Hebei Province Agricultural University of Hebei, Baoding 071001, P.R.China [2]Agricultural Department of Hebei Province, Shijiazhuang 050011, P.R. China

出　　处：《Agricultural Sciences in China》2006年第12期938-943,共6页中国农业科学（英文版）

基　　金：Acknowledgements This work was supported by the National Natural Science Foundation of China （30170602）.

摘　　要：Amplified fragment length polymorphism （AFLP） analysis was carried out in Thatcher, near isogenic lines （NILs） canting different genes conferring resistance against wheat leaf rust, and TcLr45 × Thatcher F2 progenies were used to develop markers for Lr45 gene. Sixty AFLP primer combinations were screened and most of them provided clear amplification products, 31 primer combinations displayed polymorphism of TcLr45 in 23 NILs. Two AFLP markers closely linked to the gene Lr45 were acquired： P-AGG/M-GAG261bp, which was found closely linked to the Lr45 locus at a distance of 0.6 cM on one side, and P-ACA/M-GGT105bp, which was found at a distance of 1.3 cM on the other side. The specific hands were cloned and subsequently sequenced. The 261-bp fragment produced by P-AGG/M-GAG showed 86% similarity with the sequence of Vulgate Hort I gene; the 105-bp fragment produced by P-ACA/M-GGT showed 96% similarity with the phosphatidylserine decarboxylase gene of the Triticum monococcum. Both included an open reading frame （ORF）.Amplified fragment length polymorphism （AFLP） analysis was carried out in Thatcher, near isogenic lines （NILs） canting different genes conferring resistance against wheat leaf rust, and TcLr45 × Thatcher F2 progenies were used to develop markers for Lr45 gene. Sixty AFLP primer combinations were screened and most of them provided clear amplification products, 31 primer combinations displayed polymorphism of TcLr45 in 23 NILs. Two AFLP markers closely linked to the gene Lr45 were acquired： P-AGG/M-GAG261bp, which was found closely linked to the Lr45 locus at a distance of 0.6 cM on one side, and P-ACA/M-GGT105bp, which was found at a distance of 1.3 cM on the other side. The specific hands were cloned and subsequently sequenced. The 261-bp fragment produced by P-AGG/M-GAG showed 86% similarity with the sequence of Vulgate Hort I gene; the 105-bp fragment produced by P-ACA/M-GGT showed 96% similarity with the phosphatidylserine decarboxylase gene of the Triticum monococcum. Both included an open reading frame （ORF）.

关 键 词：wheat leaf rust AFLP Lr45 gene localization 

分 类 号：S512.1[农业科学—作物学]