日循环高温对肉鸡线粒体活性氧产生量、钙泵活性及胸肌品质的影响  

The Effect of Cyclic High Temperature on Mitochondrial ROS Production, Ca^(2+)-ATPase Activity and Breast Meat Quality of Broilers

作　　者：冯京海[1] 张敏红[1] 郑姗姗[1] 谢鹏[1] 李军乔 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100094 [2]河北省邢台农业学校,邢台054000

出　　处：《畜牧兽医学报》2006年第12期1304-1311,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：通过研究日循环高温对肉鸡线粒体活性氧产生量、钙泵活性的影响,探讨高温影响肉鸡胸肌品质的机制。研究发现,日循环高温显著升高肝脏线粒体H2O2的产生量(P<0.05),对胸肌线粒体H2O2产生量有提高趋势(P=0.067 4),导致肝脏和胸肌脂质过氧化(P<0.05),并抑制胸肌和肝脏线粒体钙泵活性(P<0.05),影响肌纤维膜的完整性,使血液中肌酸激酶(CK)和乳酸脱氢酶(LDH)活性升高(P<0.01,P<0.05),表明日循环高温影响肌浆钙离子调控功能,导致肌肉中乳酸含量升高(P=0.070 3),最终使肉鸡屠宰后胸肌pHi显著降低(P<0.05),胸肌L*、滴水损失和剪切力显著升高(P<0.01)。配对组与适温组在线粒体H2O2产生量、钙泵活性、脂质过氧化、乳酸含量以及胸肌pH、L*、滴水损失等指标上无显著差异(P>0.05)。结果表明,日循环高温引起肉鸡氧化应激,影响线粒体钙离子转移系统功能,增加胸肌乳酸的积聚,使屠宰后胸肌pH下降速度加快,导致胸肌蛋白质变性,影响胸肌肉色和持水力,日循环高温对肉鸡线粒体功能及肉品质的影响与高温降低肉鸡采食量无关。The present study was conducted to determine the adverse effects of cyclic high temperature on mitochondrial function and breast meat quality of broilers. One hundred eighty 4-weekold broilers were allocated to three treatments according to following design： 25 ℃, ad lib. feeding （Control） ; 25 ℃, pair-feeding with the high cyclic group （PF） ; and 28 ℃/34 ℃ daily cyclic high temperature, ad lib. feeding （Cyclic）. Broilers were kept in three environment control chambers for three weeks. Heat exposure significantly elevated hepatic mitochondrial H2O2 production and muscular and hepatic lipid peroxidation, significantly inhibited muscular and hepatic mitochondrial Ca^2＋-ATPase activity. Increased plasma CK and LDH were found under cyclic high ambient temperature, which indicated that the muscle intracellular free Ca^2＋ was increased and muscle cell membrane integrity was damaged. At 49 day, Consistent with cyclic high temperature induced alterations in mitochondrial function were changes in breast muscle glycolytic metabolism as indicated by lower muscle pH immediately postslaughter （pHi）, and higher drip loss, L^＊ and shear force. At 25 ℃, restrict feeding had no significant effects on mitochondrial function, muscle pH, L^＊ , and drip loss, which indicated that effects of cyclic high temperature on mitochondrial function and breast meat quality of broilers were not associated with declined in feedrintake.

关 键 词：日循环高温 线粒体 活性氧 钙泵 胸肌品质 肉鸡 

分 类 号：S831.5[农业科学—畜牧学]