奥地利黑松组培苗生根技术研究  

Study on Inducing Roots from Pinus nigra var.austriaca Plantlets

作　　者：王小玲[1] 樊军锋[2] 余发新[1] 王娟娟[2] 

机构地区：[1]江西省科学院生物资源研究所,江西南昌330029 [2]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2007年第1期63-66,共4页Journal of Northwest Forestry University

基　　金：国家林业局"948"项目(2000-04-03)

摘　　要：本试验采用了试管内生根和试管外生根两种方法:(1)把长度>3 cm的奥地利黑松无根组培苗接种到含有不同激素和碳源的1/2DCR、WPM、1/2GD培养基中生长;(2)把长度>5cm的奥地利黑松无根组培苗,蘸取不同浓度的IBA、IAA、NAA溶液,转移至营养土中生长。结果表明:试管内生根优于试管外生根,试管内生根的最佳培养基为WPM,并添加NAA0.02mg.L-1+IBA0.5 mg.L-1+20 g.L-1蔗糖,;其生根率为55%。There were two methods in inducing roots, rooting in-tube and rooting out -tube. In the same culture condition , ① Select bigger than 3 centimeters Pinus nigra var. austriaca plantlets vaccinatesed 1/2DCR ,WPM ,1/ 2GI） mediums to induce roots, which includes different kinds of hormones and sucroses; （2）Select bigger than 5 centimeters Pinus nigra var. plantlets dipped in different concentration IBA ,IAA ,NAA solution, then shifts them to the nutrition soil to grow. The result showed that rooting in-tube was better and the best medium was WPM, adding NAA 0. 01 mg ·L^-1 ＋ IBA0.5 mg ·L^-1＋ sucrose 20 mg ·L^-1,on which the rate of rooting was 55%.

关 键 词：奥地利黑松 组培苗 试管内生根 试管外生根 

分 类 号：S791.24[农业科学—林木遗传育种]