反相高效液相色谱-荧光法检测血清中盐酸曲马多  

Determination of Tramadol Hydrochloride in Serum by HPLC with Fluorescence Detector

作　　者：孙国庆[1] 朱颖[1] 谷丽[2] 平其能[1] 

机构地区：[1]中国药科大学药剂学教研室,南京210009 [2]中国药科大学1995届

出　　处：《中国药科大学学报》1996年第8期476-479,共4页Journal of China Pharmaceutical University

摘　　要：采用反相高效液相色谱-荧光法检测血清中盐酸曲马多的浓度。色谱柱为Zorbax CN(4.6 mm×250 mm ID),流动相为甲醇-水-磷酸(90∶10∶0.35),柱温60℃,流速1.8 ml/min,激发波长(Ex)275 nm,发射波长(Em)302 nm,进样体积20μl。在本实验条件下,血清对测定无干扰,盐酸曲马多及其代谢物之间分离度大于1.5,理论塔板数按盐酸曲马多计算大于3000。盐酸曲马多在10～800 ng/ml浓度范围内具有良好的线性关系,标准曲线方程为C=10.3364+0.01883A,其最小检测限可达10ng/ml,平均回收率为(99.66±3.46)%。本法测定盐酸曲马多结果准确,重现性好。A HPLC method with fluorescence detector for determining tramadol hydrochloride concentration in serum was developed. The chromatographic conditions were: mobile phase, CH_3OH-H_2O-H_3PO_4(90: 10: 0. 35); column Zorbax-CN(4. 6 min×250 mm ID); flow rate 1.8 ml/min; column temperature 60C; fluorecence detector,Ex275 nm and Era304nm. The average recovery of tra madol was 99.66%. The method was simple, rapid, sensitive and reproducibile. Its linear range was 10～800 ng/ml(r=0.9996). The method could be used for tramadol hydrochloride assay and kinetits study.

关 键 词：盐酸曲马多 高效液相色谱 

分 类 号：R971.1[医药卫生—药品]