γ射线外照射诱发人体外周血淋巴细胞DNA损伤的研究  

Effects of DNA Damage in Peripheral Blood Lymphocytes of human Induced by γ-Rays Irradiation.

作　　者：姜林[1] 杨玉青[1] 刘国平[1] 许云书[1] 高清祥[2] 

机构地区：[1]中国工程物理研究院核物理与化学研究所,四川绵阳621900 [2]兰州大学生命科学学院

出　　处：《中国辐射卫生》2006年第4期396-398,共3页Chinese Journal of Radiological Health

基　　金：中国工程物理研究院科学技术基金资助项目(20050547)

摘　　要：目的研究γ射线外照射诱导正常人血淋巴细胞DNA损伤的剂量效应关系。方法以正常人体外周血淋巴细胞为材料,采用单细胞凝胶电泳技术(Single cell gel electrophoresis,SCGE,又称彗星电泳),研究了不同剂量γ射线外照射诱导正常人血淋巴细胞DNA瞬时损伤的情况。结果0、0.1、0.5、12、和3 Gyγ射线外照射淋巴细胞后,彗尾DNA含量(y)与受照剂量(x)关系为y=-1.1063x2+10.398x+3.5362,Olive矩(y)与受照剂量(x)关系为y=-0.0345x2+3.5282x+0.6542。结论γ射线外照射正常人血淋巴细胞DNA有明显的损伤作用,所致原初损伤与受照剂量呈良好的线形平方关系,单细胞凝胶电泳技术有望成为新一代生物剂量计。Objective To investigate the dose- effect curve of the human Peripheral Blood Lymphocytes irradiated by γ- rays. Methods The immediate irradiation- caused DNA damage in the human Peripheral Blood Lymphocytes irradiated with γ- rays was investigated. The single cell gel electrophoresis（SCGE,Comet Assay） were used to detect the extent of DNA damage. Resuits After 0 Gy ,0.1Gy,0.5 Gy,1 Gy,2 Gy and 3 Gyγ- rays irradiation, the curve of dose（x） to content of DNA in tail（y） is y = - 1.1063x^2 ＋ 10.398x ＋ 3.5362, while the curve of dose（x） to tail moment（y） is y= -0.0345x^2 ＋ 3.5282x ＋ 0.6542. Conclusion The results indicated that the content of DNA in tail and tail moment for the immediate DNA damage caused by irradiation had a psitively binomial curve correlation with the irradiation dose in the dose range of 0 ~ 3 Gy, and SCGE might be used as a new biological dosimeter.

关 键 词：单细胞凝胶电泳 DNA损伤 Γ射线 

分 类 号：R135[医药卫生—劳动卫生]