hTERT和PCNA在良恶性胸腹水细胞中的表达及临床意义  

Expression of human telomerase reverse transcriptase and proliferating cell nuclear antigen in pleural and peritoneal effusion

作　　者：吴丽颖[1] 刘邦荣[2] 陆军[2] 凌明德[1] 陈洁[2] 李朋[1] 

机构地区：[1]安徽省淮北市人民医院消化内科,淮北235000 [2]安徽省淮北市人民医院病理科,淮北235000

出　　处：《第三军医大学学报》2007年第2期154-156,共3页Journal of Third Military Medical University

基　　金：淮北市科技计划项目(040143)~~

摘　　要：目的探讨端粒酶逆转录酶(humantelomerasereversetranscriptase,hTERT)和增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA)在良恶性胸、腹水中的表达及临床意义。方法应用免疫细胞化学S-P法分析46例恶性胸、腹水患者、21例良性胸、腹水患者的胸、腹水脱落细胞hTERT和PCNA的表达状况。结果hTERT和PCNA在46例恶性胸、腹水中的阳性表达率分别为84.8%和63.0%,良恶性胸、腹水中反应性间皮细胞均未见hTERT和PCNA表达。hTERT和PCNA表达呈明显正相关(r=0.30,P<0.05)。hTERT和PCNA联合检测较二者单独检测敏感性提高至89·1%。结论hTERT的表达对于良恶性胸、腹水的鉴别诊断具有一定的辅助意义,hTERT联合PCNA检测较单独检测及细胞学检测敏感性进一步提高。Objective To explore the diagnostic value of human telomerase reverse transcriptase （hTERT） and proliferating cell nuclear antigen （PCNA） in pleural and peritoneal effusion. Methods The expressions of hTERT and PCNA were detected by immunocytochemistry in 46 malignant and 21 benign serous specimens. Results hTERT and PCNA were detected respectively in 39 and 29 of 46 malignant specimens, but no expressions in reactive mesothelial cells in all serous specimens, hTERT correlated with PCNA （r = 0. 30, P 〈 0. 05 ）. The combined detection of hTERT and PCNA increased the sensitivity to 89.1%. Conclusion hTERT is conducive to diagnosis of serous effusion by cytologic examination. The sensitivity was in- creased by combined detection of hTERT and PCNA, as compared with cytologic examination, detection of hTERT or PCNA alone.

关 键 词：端粒酶逆转录酶 增殖细胞核抗原 胸腹水 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] R447[医药卫生—基础医学]