结肠癌组织肿瘤相关巨噬细胞VEGF-C/D的表达及与淋巴结转移的关系  

Correlation between VEGF-C/D expressions in tumor associated macrophages and lymph node metastasis in colon cancer

作　　者：李焱[1] 蒋勇[1] 史立群[2] 史爱学[2] 彭亦良[1] 梁后杰[1] 

机构地区：[1]第三军医大学西南医院肿瘤科,重庆400038 [2]第三军医大学西南医院病理学研究所,重庆400038

出　　处：《第三军医大学学报》2007年第2期157-159,共3页Journal of Third Military Medical University

摘　　要：目的了解结肠癌肿瘤相关巨噬细胞中VEGF-C/D的表达,探讨其对结肠癌淋巴结转移的影响及可能机制。方法采用特异的巨噬细胞标记物CD68等抗体以免疫组化染色的方法,并用双染、连续切片对比等对45例结肠癌标本中巨噬细胞VEGF-C/D的表达进行检测,同时行巨噬细胞计数,并统计分析两者间存在的关系。结果在结肠癌相关巨噬细胞中检测到两种淋巴管生成因子的表达。统计分析显示VEGF-C表达与巨噬细胞计数具有相关性。有淋巴结转移者VEGF-C阳性细胞率高于无转移组。结论结肠癌肿瘤组织中,不仅肿瘤细胞可分泌表达VEGF-C/D,肿瘤相关巨噬细胞也可表达VEGF-C/D。肿瘤相关巨噬细胞可通过诱导结肠癌局部淋巴管生成,对肿瘤的转移可起到一定的作用。Objective To discuss the correlation between the VEGF-C/D expressions in tumor associated macrophage （TAM） and lymph node metastasis in colon cancer. Methods Forty-five colon cancer samples proven pathologically to be adenocarcinoma were stained by immunohistochemical method for VEGF-C/D and CD68. The correlation of VEGF-C/D expressions, macrophage counting and lymph node metastasis were analyzed statistically. Results The expressions of VEGF-C/D were detected in TAMs. VEGF-C expression was obviously higher in the samples with lymphatic metastasis. VEGF-C expression in colon cancer was related to the number of TAMs. Conclusion TAMs are important in the lymph node metastasis, because they can express VEGF-C/D and affect the lymphangiogenesis of colon cancer.

关 键 词：VEGF—C VEGF-D 肿瘤相关巨噬细胞 结肠癌 

分 类 号：R392.2[医药卫生—免疫学] R73-37[医药卫生—基础医学]