合浦珠母贝印度家系F_1代的AFLP分析  

AFLP analysis on a familial F_1 population of pearl oyster Pinctada fucata

作　　者：王小玉[1] 喻达辉[1] 黄桂菊[1] 郭奕惠[1] 龚世园[2] 王爱民[3] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]华中农业大学水产学院,湖北武汉430072 [3]海南大学海洋学院,海南海口570228

出　　处：《中国水产科学》2007年第1期52-58,共7页Journal of Fishery Sciences of China

基　　金：国家"863"重大科研项目(2002AA603022);广东省科技计划项目(2002B2150101);广东省自然科学基金项目资助(037148)

摘　　要：用AFLP标记对合浦珠母贝(Pinctada fucata)印度家系的亲本及其子一代(F1)进行了遗传分析。27对引物共扩增出1 013个位点,其中835个位点在F1代分离,178个位点不分离,多态位点比例82.4%。分离位点中,符合孟德尔分离规律的位点458个,占分离位点数的54.9%。偏孟德尔分离规律的位点377个,占分离位点数的45.1%。分离比为3∶1的位点共有482个,占分离位点数的57.7%,符合孟德尔分离规律的位点251个,占52.1%(占分离位点数的30.1%);分离比为1∶1的位点共有353个,占分离位点数的42.3%,符合孟德尔规律的位点207个,占58.6%(占分离位点数的24.8%)。半数以上的标记符合孟德尔遗传规律。表明AFLP标记适合于合浦珠母贝的遗传图谱构建。[中国水产科学,2007,14(1):52-58]The pearl oyster Pinctada fucata distributed in the Pacific, Atlantic and Indian Oceans is the most important species cultured for pearl production. It has been cultured along the coasts of Guangdong, Guangxi and Hainan Provinces in China's Mainland since 1960＇ s. Yet there were large scale mortalities occurred in culture practices in the recent years. To resolve this problem, a selective breeding program has been initiated, including family selection. In this study the polymorphism and segregation of AFLP marke, rs in the F1 generation of an Indian P. fucata family were investigated using 27 selective primer combinations. A total of 1 013 loci were generated, among which 835 were polymorphic in F1 generation, accounting for 82.4% ,and 178 loci were monomorphic, accounting for 17.6%. Among 835 segregating loci, 458 loci （54.9%） segregated in Mendelian ratio while 377 loci （45.1% ） segregated in distortion as indicated by Chi-square test （ P 〈 0.05）. There were 482 （57.7 % ） and 353 （42.3 % ） loci segregating in the ratios of 3 ： 1 and 1 ： 1,respectively. The numbers of loci segregating in Mendelian law were 251 （52.1%） and 207 （58.6%） for 3：1 and 1：1 loci, respectively. The number of loci segregating in Mendelian ratio is higher than that segregating in distortion. The results demonstrate that AFLP is highly efficient and can be used to construct genetic linkage map for pearl oyster P. fucata.

关 键 词：孟德尔分离规律 AFLP标记 合浦珠母贝 家系 

分 类 号：Q75[生物学—分子生物学]