利用赭曲霉NG1203羟基化齐墩果酸的研究  

Hydroxylation of oleanolic acid by hydroxylase from Aspergillus ochraceus NG1203

作　　者：王晋宇[1] 欧阳嘉[1] 沈珈琦[1] 范伟平[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《生物加工过程》2006年第4期51-55,共5页Chinese Journal of Bioprocess Engineering

摘　　要：考察了利用赭曲霉Aspergillus ochraceusNG1203进行C11α-羟基化齐墩果酸生化反应的条件。得出了菌株摇瓶培养最佳培养基配方(g.L-1):葡萄糖20,玉米浆25,酵母膏3,K2HPO41.5,pH 6.0。菌株培养20 h,加入2 mg.L-1的齐墩果酸利于诱导羟基化酶的产生。菌株在28℃下以150 r.min-1振荡培养24 h,加入底物的乙醇溶液,使转化液中齐墩果酸的初始质量浓度达100 mg.L-1,转化液中乙醇体积分数最终达3%。经96 h转化,齐墩果酸转化率可达到10.12%。通过HPLC1、H NMR和13C NMR分析,结果表明产物为C11α-羟基齐墩果酸。Microbial transformation of oleanolic acid to E11α-hydroxyl derivative was investigated by cells of Aspergillus ochraceus NG1203. The optimum culture medium contains （g·L^-1）： glucose 20.0, corn steep liquor 25.0, yeast extract 3.0, K2HPO4 1.5 mg·L^-1 of substrate was added to the culture after 20 h could induce the hydroxylase secretion. After the strain incubated in 500 mL shake flasks with 100 ml medium at 28 ℃, 150 r·min^-1 for 24 h,100 mg·L^-1 oleanolic acid was added. The aqueous medium included 3% ethanol in the end of the culture. The biotransformation yield was attained to 10.12% after 96 h using the free Aspergillus ochraceus NG1203 cells. The purified product was identified as C11α-hydroxyl oleanolic acid by HPLC,^1H NMR,and ^13C NMR.

关 键 词：齐墩果酸 羟基化反应 赭曲霉NG1203 

分 类 号：Q554.02[生物学—生物化学]