盾壳霉产几丁质酶培养条件的研究  

Study on Culturing Condition of Chitinase Produced by Coniothyrium Minitans

作　　者：马丽娜[1] 高俊明[1] 李欣[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2006年第4期329-331,334,共4页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：山西省自然科学基金(20041096)

摘　　要：通过在SMCS培养液中加不同的碳源、氮源以及双碳源,改变培养时间、温度及培养液的pH值,研究了盾壳霉产几丁质酶的培养条件。结果表明：盾壳霉接种在改良的SMCS液体培养基中置于恒温摇床(200 r·min^1．20℃)上培养15 d,可诱导产生大量的几丁质酶。改良的SMCS培养液配方：KH_2PO_4680 mg,K_2HPO_4870 mg,KC1200 mg,NH_4NO_3lg,MgSO_4·7H_2O_200 mg,CaCl_2 200 mg．FeSO_4 2 mg．znSO_4 2 mg,MnSO_4 2mg,葡萄糖5 g,几丁质5 g,加水1000 mL,调pH值到6．5。In this study the different carbon source, nitrogen source and double carbon source are mixed in the SMCS nutrient fluid. The culturing time, the temperature and the pH are changed in order to study culturing condition of chitinase pro duced by Coniothyrium minitans. The result indicated that Coniothyrium minitans on the improved SMCS nutrient fluid at the constant temperature on the skakier （200 r · min^-, 20 ℃） about 15 days, mayinduce massive Chitinase. The improved SMCS nutrient fluid formula is, KH2PO4 680 mg, K2 HPO4 870 mg, KCl 200 mg, NH4NO3 1 g, MgSO4 · 7H2O 200 mg, CaCl2 200 mg, FeSO4 2 mg, ZnSO4 2 mg, MnSO4 2 mg, glucose 5 g, chitin 5 g, water 1 000 mL, adjusting the pH to 6.5.

关 键 词：盾壳霉 几丁质酶 培养条件 

分 类 号：S435.655[农业科学—农业昆虫与害虫防治]