大肠埃希菌连续分离株耐药性与三类整合酶基因的研究  

A statistical study upon three types of integrase genes related with the antibiotic resistance of continuously isolated Escherichia Coil strains

作　　者：王继东[1] 钱小毛[2] 郁敏[1] 

机构地区：[1]江南大学附属医院无锡市第五人民医院,江苏无锡214073 [2]绍兴第二医院,浙江绍兴312000

出　　处：《世界感染杂志》2006年第6期520-522,共3页World Journal of Infection

基　　金：无锡市克隆遗传技术研究所CL0N-GEN细菌耐药基因专项资金资助（N020060301WX）

摘　　要：目的 了解大肠埃希菌连续分离株整合酶基因存在情况和耐药性。方法 应用聚合酶链反应（PCR）法对耐药的大肠埃希菌进行int I1、int I2、int I3三类整合酶基因检测与分析。结果 PCR结果显示：整合酶基因检测68株中int I1基因阳性47株（69．1％），int I2基因阳性1株（1．5％），无int I3基因阳性。结论 整合子在细菌耐药性播散过程中起非常重要的作用，整合子使细菌能够很快获得新的耐药基因以适应环境要求。Objective To investigate the distribution and drug-resistance of integrase genes related with the antibiotic resistance of continuously isolated Escherichia Coli strains. Methods To analyze and detect int I1, int I2 and int I3 integrase genes in the antibiotic-resistant E. coli by applying PCR test kits. Results in 68 strains of E. coli isolated from patients, 47 strains were positive to the int I1 gene （69.1%）, 1 was positive to int I2 gene （1.5%） and negative to int I3 gene. Conclusion Intergron is the most important factor assisting the spread of the antibiotic resistance strains of E. coli. It enables E. coli to acquire new drug-resistance genes to quickly adjust to the environment.

关 键 词：细菌耐药 大肠埃希菌 整合酶基因 

分 类 号：R378.2[医药卫生—病原生物学] R-33[医药卫生—基础医学]