奶牛附红细胞体病三种检测方法的比较研究  

The comparative study between three detection methods of eperythrozoonosis in cows

作　　者：路义鑫[1] 宋铭忻[1] 王君伟[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《东北农业大学学报》2006年第6期783-787,共5页Journal of Northeast Agricultural University

基　　金：国家"十五"重大科技专项子课题(210044)

摘　　要：对黑龙江省5个奶牛小区中的近444头奶牛分别运用悬滴法、直接涂片法、染色法进行了附红细胞体感染情况检测。结果表明,悬滴法和直接涂片法的感染率为68.2%￣78.6%,姬姆萨染色法为54.5%￣64.7%、吖啶橙染色法为31.8%￣50.8%,悬滴法和直接涂片法的阳性率较高,染色法的结果更接近于实际。建议临床诊断应尽量采用染色法,并结合流行病学、临床症状、治疗等情况进行综合判断。Detect the eperythrozoon infection from 444 cows of 5 cow areolae in Heilongjiang Province, in the means of hanging drop method, the method of direct smear and Giemsa＇s staining method. As a result, the infection rate detected by the hanging drop method and the method of direct smear is 68.2%-78.6%, the Giemsa＇s staining method is 54.5%-64.7% and acridine orange staining method is 31.8%-50.8%. The positive proportion by the hanging drop method and the method of direct smear is higher, but the result by staining method is more closed to the fact. Suggest the clinic diagnosis Should adopt the staining method as far as possible, and with the epidemiology at the same time, clinical symptom and the cure etc.

关 键 词：奶牛 附红细胞体 检测 

分 类 号：S858.23[农业科学—临床兽医学]