矮生一品红组织培养快繁体系的建立  

Establishment of tissue culture and rapid propagation system for dwarf Euphorbia pujcher ima

作　　者：尹英敏[1] 朴日子[1] 曹后男[1] 郑仙霞[1] 陈蕾[1] 

机构地区：[1]延边大学农学院园艺系,吉林龙井133400

出　　处：《延边大学农学学报》2006年第4期293-297,共5页Agricultural Science Journal of Yanbian University

摘　　要：以矮生一品红的幼叶、幼茎、茎芽为外植体,研究了影响丛芽诱导、增殖、生根壮苗的组培快繁的主要因素.结果表明:茎段、茎芽是诱导丛芽的较好试材.适合诱导胚性愈伤组织和芽分化的培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L;适合丛芽增殖成苗的培养基为MS+6-BA 1.0 mg/L+IAA 0.1 mg/L,继代增殖培养40 d的平均增殖倍数为11;适合生根壮苗的培养基为1/2 MS+IBA 0.9 mg/L,开始生根天数为8 d,生根率可达85%.采用河砂和营养土两步炼苗,移栽成活率可达90%以上.The young leaves, young stem segments and stem segments with buds were used as explants to study the mainly factors which affected the cluster buds induction, multiplication and seedlings rooting and transplant of tissue culture and rapid propagation. The results showed that the stem segments and stem bud were better for inducing cluster buds. MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.1 mg/L was suitable for inducing embryoid callus and cluster buds ; MS ＋ 6-BA 1.0 mg/L ＋ IAA 0.1 mg/L was suitable for cluster buds multiplication, and the average multiplication was 11 when cultured 40 days; 1/2 MS ＋ IBA 0.9 mg/L was suitable for rooting, which started rooting eight days later, and the rooting was up to 85 %. River sand and humus were used in two successive steps,and the survival rates was more than 90 %.

关 键 词：一品红 组织培养 快繁体系 激素 

分 类 号：S685.23[农业科学—观赏园艺]