2-酮戊二酸对水稻根部碳-氮代谢重要酶的活性影响  

Effects of 2-Oxoglutarate on Main Enzymes of Carbon-Nitrogen Metabolism in Roots of Rice(Oryza sativa L.) Seedings

作　　者：鲍世颖[1] 袁永泽[1] 周志鹏[1] 张楚富[1] 

机构地区：[1]武汉大学生命科学学院/植物发育生物学教育部重点实验室,湖北武汉430072

出　　处：《武汉大学学报（理学版）》2006年第6期763-766,共4页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30270130)

摘　　要：2-酮戊二酸是碳-氮代谢的重要调控因子,在培养液中添加2-酮戊二酸可以促进水稻幼苗根部碳-氮代谢酶的活性.为了确定2-酮戊二酸是否与这些酶之间存在直接作用(体内实验),取正常培养的水稻幼苗材料,在上述酶的测活体系中分别添加2-酮戊二酸,并进行酶活性测定(体外实验).实验结果表明:2-酮戊二酸可以促进水稻幼苗根组织提取液中GS,HXK的活性,而对GOGAT,ICDH没有明显的影响.上述结果说明2-酮戊二酸作为一种效应物可能与GS、HXK结合,从而在体内、体外都促进了它们的活性.In carbon and nitrogen metabolisms of higher plants, 2-oxoglutarate is an important regulation effector. Some experiments have already showed that 2-oxoglutarate could promote enzymes of carbon and nitrogen metabolisms in roots of rice seedlings roots（experiment in vivo）, for example, HXK, GS, GOGAT and ICDH. To conform whether direct interaction between 2-oxoglutarate and these enzymes, we made enzyme extracts from rice seedling roots in normal culture condition （free 2-oxoglutarate）, and the different concentrations of 2-oxoglutarate were added to the reaction systems of the above enzymes, and determined their activities （experiment in vitro）, respectively. The results showed that the activities of GS,HXK were promoted by 2-oxoglutarate, hut GOGAT and ICDH were not affected. This suggested that 2-oxoglutarate, which was neither their substrate nor their product, maybe as an effector of GS and HXK to promote their activities both in vivo and in vitro.

关 键 词：2-酮戊二酸 水稻 碳-氮代谢 酶 

分 类 号：Q946[生物学—植物学]