逆灸“关元”穴对大鼠更年期血浆、子宫一氧化氮合酶的影响  

Preventative Moxibustion of "Guanyuan"(CV 4) on NOS Activity in the Blood and Uterus Tissue of Menopausal Rats

作　　者：解秸萍[1] 李晓泓[1] 张露芬[1] 翟景慧[1] 张惠[1] 李蔚[1] 贾君君[1] 农艳[1] 陈旭[1] 

机构地区：[1]北京中医药大学针灸学院,北京100029

出　　处：《针刺研究》2006年第6期351-354,共4页Acupuncture Research

基　　金：国家自然科学基金资助项目(No.90209026)

摘　　要：目的:通过生物化学途径观察逆灸对大鼠更年期机体的影响。方法:选用SD雌性大鼠80只,其中4月龄10只,为年轻正常组;10月龄70只,根据阴道细胞涂片按动情周期随机分为7组,每组10只。这7组分别为逆灸12月龄组、逆灸14月龄组、逆灸16月龄组、12月龄对照组、14月龄对照组、16月龄对照组及10月龄对照组。逆灸组在10月龄时预以艾炷灸“关元”穴,每次1壮,每周2次,共灸8周。分别于12、14、16月龄时与同月龄对照组及年轻正常组、10月龄对照组一起取血浆、子宫,以硝酸还原酶法检测一氧化氮合酶(NOS)活性。结果:①大鼠更年期血浆NOS活性的变化及逆灸“关元”穴对其影响:对照各组与年轻正常组比较,14月龄组NOS活性明显下降,P<0.01;逆灸各组与年轻正常组比较无显著性差异,P>0.05;逆灸14月龄组比同月龄对照组NOS活性增加,P<0.05。②大鼠更年期子宫NOS的变化及逆灸“关元”穴对其影响:对照各组与年轻正常组比较,14、16月龄组NOS活性明显降低,有非常显著性差异,P<0.01;逆灸各组与年轻正常组比较,14、16月龄组NOS活性降低,有显著性差异,P<0.05;逆灸16月龄组比16月龄对照组NOS明显升高,P<0.05。结论:逆灸能升高更年期大鼠血浆及子宫中NOS活性,其早期类似于预处理效应,中后期表现为明显的调节效应,类似预适应的延迟效应,体现了逆灸“关元”穴的保健作用。Objective： To research the effect of preventative moxibustion （PM） of ＂Guanyuan＂ （CV 4） on nitric oxide synthase （NOS） activity in the plasma and uterus tissue in menopausal rats. Methods： A total of 70 female SD rats （aged 10 months） were randomized into 10-months （mo） control （con）, 12-mo-con, 14-mo-con and 16-mo-con, 12-mo-PM, 14-mo-PM and 16-mo-PM groups with 10 rats in each group. In addition, other 10 female SD rats aged 4 mo were used as young-con group. In rats of 3 PM groups, moxibustion （one moxa-cone/time） was applied to ＂Guanyuan＂ （CV 4） twice a week, 8 weeks altogether. At the end of experiments all the rats were decapitated for collecting blood samples and uterus tissues. NOS activities in plasma and the homogenized uterus tissue supernate were detected with nitrate reductase method. Results： In comparison with youngcon group, NOS activities of both plasma and uterus tissue in 14-mo-con group, and those of uterus in 16-mo-con group, 14-mo-PM and 16-mo-PM groups decreased significantly （P〈0.05, 0.01 ）. Comparison between 14-rap-con and 14-mo-PM groups and between 16-mo-con and 16-mo-PM groups indicated that plasma NOS activity of 14-mo-PM group and uterus NOS activity of 16-mo- PM group were significantly higher than those of the former groups, suggesting that preventive moxibustion could inhibit the decline of NOS levels in blood and uterus tissue. Conclusion ： Preventive moxibustion of CV 4 can effectively relieve the decline of plasma and/or uterus NOS levels in menopausal rats.

关 键 词：逆灸 更年期 一氧化氮合酶(NOS) 

分 类 号：R245.81[医药卫生—针灸推拿学]