肾素-血管紧张素-醛固酮系统中不同组分在脂肪组织中的表达  

Expression of Renin-Angiotensin-Aldosterone System in Human Adipose Tissues

作　　者：张雪晗[1] 曾正陪[1] 李汉忠[2] 周亚茹[1] 张晶[1] 童安莉[1] 阎朝丽[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院内分泌科 [2]Department of Urology,PUMC Hospital,CAMS and PUMC,Beijing100730

出　　处：《中国医学科学院学报》2006年第6期766-769,共4页Acta Academiae Medicinae Sinicae

基　　金：国家"十五"科技攻关计划课题(2004BA720A29)~~

摘　　要：目的观察肾素-血管紧张素-醛固酮系统(RAAS)的不同组分在人皮下、肾脏和肾上腺周围脂肪组织中的表达,探讨不同脂肪组织中RAAS各组分的特点。方法提取12例皮下脂肪、12例肾周脂肪和9例肾上腺周围脂肪总RNA,逆转录-聚合酶链反应方法进行血管紧张素原(AGT)、肾素(Renin)、血管紧张素转化酶(ACE)、血管紧张素转化酶2(ACE2)、血管紧张素Ⅱ受体1(AT1)、血管紧张素Ⅱ受体2(AT2)、醛固酮合成酶(CYP11B2)的mR-NA表达。结果皮下、肾脏和肾上腺周围脂肪组织中均存在AGT、ACE、ACE2、AT1和AT2mRNA表达,无CYP11B2mRNA表达。肾素mRNA仅在肾周和肾上腺周围脂肪组织中表达,其在肾周的表达显著高于肾上腺周围脂肪组织(P<0·05)。ACE、ACE2mRNA在肾周脂肪组织中的表达显著高于皮下脂肪组织(P<0·05)。ACEmRNA在3种脂肪组织中的表达均显著高于ACE2(P<0·05)。在肾上腺周围脂肪组织中,AT2mRNA的表达显著高于AT1(P<0·05)。结论脂肪组织中存在局部肾素-血管紧张素系统,但无醛固酮合成。Objective To compare the mRNA expression of renin-angiotensin-aldosterone system in human subcutaneous and visceral adipose tissues. Methods Total RNA was extracted from 12 human subcuta- neous adipose tissues, 12 perirenal adipose tissues and 9 periadrenal adipose tissues..The expressions of angiotensinogen （AGT）, renin, angiotensin converting enzyme （ACE）, angiotensin converting enzyme 2 （ACE2） , angiotensin Ⅱ receptor type 1 （ AT1 ） , angiotensin Ⅱ receptor type 2 （ AT2 ） , CYP11B32, and their internal reference glyceraldehyde phosphate （GAPDH） were studied by reverse transcription-polymerase chain reaction. The ratios of each target genes were used to evaluate the expression levels of AGT, renin, ACE, ACE2, AT1, AT2, and CYP11B2 in different adipose tissues. Results The mRNA expressions of AGT, ACE, ACE2, AT1, and AT2 were detected in human subcutaneous, perirenal, and periadrenal adipose tissues. However, CYP11 B2 mRNA expression was not found in these three adipose ti- ssues. The mRNA expressions of renin was only detected in perirenal and periadrenal adipose tissues, which was significantly higher in perirenal adipose tissues than in periadrenal adipose tissues （ P 〈 0. 05 ）. The mRNA expressions of ACE and ACE2 in perirenal adipose tissues were significantly higher than that in subcutaneous adipose tissues （ P 〈0. 05 ）. The mRNA expressions of ACE were significantly higher than that of ACE2 in subcutaneous, perirenal, and periadrenal adipose tissues （ P 〈 0. 05 ）. The mRNA expressions of AT1 were significantly lower than that of AT2 in periadrenal adipose tissues （ P 〈 0. 05 ）. Conclusion Local renin-angiotensin system exists in the adipose tissues; however, aldosterone is not synthesized in the adipose tissues.

关 键 词：肾素-血管紧张素-醛固酮系统 皮下脂肪组织 肾周脂肪组织 肾上腺周围脂肪组织 

分 类 号：R589.2[医药卫生—内分泌]