大鼠口服红花水提物和红花黄色素后羟基红花黄色素A的排泄研究  

An Analysis of Hydroxysafflor Yellow A Excretion of Rats After Oral Administration of Safflower Extracts and Safflor Yellow

作　　者：张朝阳[1] 盛彧欣[1] 李翼[1] 张金兰[1] 于治国[2] 

机构地区：[1]中国医学科学院药物研究所,北京100050 [2]沈阳药科大学药学院,沈阳110016

出　　处：《世界科学技术-中医药现代化》2006年第6期107-112,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科技部新技术新方法的研究(Ke2004-03):羟基红花黄色素A在大鼠体内的代谢研究

摘　　要：建立简便的高效液相色谱法(HPLC)测定大鼠口服红花水提物和红花黄色素后尿、粪和胆汁中主要活性成分羟基红花黄色素A(HSYA)的含量。色谱柱为Hypersil BDS-C18(250×4．6mm,5μm),流动相为乙腈-0．3%冰醋酸水溶液梯度洗脱,检测波长320nm,葛根素或对羟基苯甲醛为内标。尿、粪和胆汁中的HSYA的日内精密度小于3．1%,日间精密度小于5．8%。提取回收率为79．2%～102．1%。该方法首次研究了大鼠口服红花水提物和红花黄色素后羟基红花黄色素A的排泄情况。In this study, a simple and reproducible high -performance liquid chromatography （HPLC） method was employed to determine hydroxysafflor yellow A （HSYA） in rat urine, feces and bile, after an oral administration of safflower extracts and safflor yellow. HPLC was performed on a Hypersil BDS -C18 column, with a gradient elution system that is made up of acetonitrile and aqueous acetic acid. Ultraviolet detection was performed at 320 nm, with puerarin and p - Hydroxybenzaldehyde as the internal standard （IS）. The intra- day and inter- day precision were registered less than 3.1% and 5.8% respectively, and the extract recovery ranged between 79.2% - 102.1% in urine, feces and bile. The effort makes the first instance of using HPLC method to work on the excretion rates of HSYA in rat urine, feces and bile, after an oral administration of safflower extract and safflor yellow.

关 键 词：红花水提物 红花黄色素 羟基红花黄色素A HPLC 排泄 

分 类 号：R969[医药卫生—药理学]