异丙酚对兔肝缺血再灌注损伤时内皮素-1和一氧化氮的影响  

Effect of propofol on endothelin-1 and nitric oxide during hepatic ischemia-reperfusion injury in rabbits

作　　者：罗遥[1] 周党军 吕毅[3] 景桂霞[1] 

机构地区：[1]西安交通大学医学院第一附属医院麻醉科,陕西西安710061 [2]咸阳市中心医院肝胆外科,陕西咸阳712000 [3]西安交通大学医学院第一附属医院肝胆外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2006年第6期582-585,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科学技术研究发展计划项目(No.2004K17-G28)

摘　　要：目的探讨异丙酚对兔在体肝缺血再灌注损伤时内皮素-1(ET-1)和一氧化氮(NO)的影响。方法40只家兔随机分为4组,每组10只,分别为缺血再灌注组(A组)、缺血再灌注加生理盐水2 mL/(kg.h)组(B组)、缺血再灌注加异丙酚8mg/(kg.h)组(C组)和假手术对照组(D组)。各组于再灌注后306、0 min测定肝组织和血浆中ET-1与NO水平以及血清谷丙转氨酶(ALT)、谷草转氨酶(AST)的水平,并进行肝组织形态学观察。结果与D组比较,A、B、C组于再灌注后30 min及60 min时,肝组织和血浆中ET-1均呈上升,但C组的上升程度明显低于同时间点的A、B两组(P<0.01);同时肝组织和血清中NO呈下降,但C组的下降程度明显低于同时间点的A、B两组(P<0.01);血清ALT、AST均升高,但C组的升高程度明显低于同时间点的A、B两组(P<0.01);肝细胞形态学发生异常改变,C组的肝脏淤血明显较A、B两组轻,肝细胞变性坏死不明显。结论异丙酚通过保护肝窦内皮,降低机体内ET-1水平,提高NO水平,改善肝脏微循环障碍,对HIRI有保护作用。Objective To investigate the effect of propofol on endothelin-1 （ET-1） and nitric oxide （NO） during hepatic ischemia-reperfusion injury （HIRI） in rabbits in vivo and to investigate its protective effect on HIRI. Methods A total of 40 rabbits were randomly divided into four groups with ten animals in each. They were ischemia-reperfusion group （group A）, ischemia-reperfusion group treated with 2 mL/（kg · h） sodium chloride （group B）, ischemia-reperfusion group treated with 8 mg/（kg · h） propofol （group C）, and sham-operation group as control （group D）. ET-1 and NO content in hepatic tissue and serum, and alanine aminotransferase （ALT）, aspartate aminotransferase（AST） content in serum were assayed 30 and 60 min after reperfusion. Morphological observation of hepatic tissues was also performed. Results In groups A, B, and C 30 and 60 min after reperfusion, ET-1 content in hepatic tissue and serum increased compared with that in group D （P 〈 0. 01 or P〈0.05）, but the increased degree in group C was much smaller than that in group A and B at the same time （P〈 0.01）. NO content in hepatic tissue and serum decreased compared with that of group D （P〈0.01 or P〈0.05）, but the decreased degree in group C was much smaller than that in group A and B at the same time（P〈0.01）. Serum ALT and AST level in group A, B, and C increased compared with that in group D （P〈0.01 or P〈0.05）, but the increased degree in group C was much smaller than that in group A and B at the same time（P〈0.01）. The morphology of hepatocytes changed abnormally. The blood stasis of hepatic tissue in group C was milder than that in group A and B, denaturalization and necrosis of hepatocyte in group C were not obvious. Conclusion Propofol can protect hepatic sinus endothelium, decrease ET-1 content and increase NO content, and thus it shows the protective effect on HIRI.

关 键 词：异丙酚 肝脏 再灌注损伤 内皮素-1 一氧化氮 兔 

分 类 号：R971.2[医药卫生—药品]